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Imaging Strategies for Studying Mammalian Oogenesis

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Oogenesis

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Abstract

Advances in biomedical imaging are now impacting the evaluation of gonads and gametes in experimental animal model systems and human clinical specimens. This chapter summarizes the rationale for both live cell and fixed sample imaging using light microscopy. Useful protocols, detailed methods, and tools for data extraction are covered for a variety of applications pertaining to the analysis of oocytes, embryos, and intact ovarian tissue.

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Acknowledgments

The authors express their gratitude to the many colleagues and friends who have worked in the lab to advance the perspectives of oocyte biology that we enjoy today. Special thanks go to Lynda McGinnis and Britta Mattson who at different ends of the spectrum contributed to the development of MTSB-XF. Finally, this chapter would not have been possible without the funding from the NIH, ESHE Fund, The March of Dimes, and the Hall Family Foundation.

Author information

Authors and Affiliations

  1. Department of Molecular and Integrative Physiology, Kansas University Medical Center, 3901 Rainbow Boulevard, Kansas City, KS, 66160, USA

    S. Darlene Limback

  2. Department of Molecular and Integrative Physiology, Center for Reproductive Sciences, Institute for Reproductive Health and Regenerative Medicine, Kansas University Medical Center, 3901 Rainbow Boulevard, Kansas City, KS, 66160, USA

    David F. Albertini Ph.D., M.S., AM, B.S.

  3. Department of Physiology, Kansas University Medical Center, 3901 Rainbow Boulevard, Kansas City, KS, 66160, USA

    David F. Albertini Ph.D., M.S., AM, B.S.

Authors
  1. S. Darlene Limback
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  2. David F. Albertini Ph.D., M.S., AM, B.S.
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Corresponding author

Correspondence to David F. Albertini Ph.D., M.S., AM, B.S. .

Editor information

Editors and Affiliations

  1. BIOGENESI, Instituti Clinici Zucchi, Via Zucchi 24, Monza, 20900, Italy

    Giovanni Coticchio

  2. University of Kansas Medical Center, 3901 Rainbow Blvd, Kansas City, 66160, USA

    David F. Albertini

  3. Ospedale S. Raffaele, Department of Ob/Gyn, University Vita-Salute, Via Olgettina 60, Milan, 20132, Italy

    Lucia De Santis

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© 2013 Springer-Verlag London

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Limback, S.D., Albertini, D.F. (2013). Imaging Strategies for Studying Mammalian Oogenesis. In: Coticchio, G., Albertini, D., De Santis, L. (eds) Oogenesis. Springer, London. https://doi.org/10.1007/978-0-85729-826-3_1

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  • DOI: https://doi.org/10.1007/978-0-85729-826-3_1

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  • Publisher Name: Springer, London

  • Print ISBN: 978-0-85729-825-6

  • Online ISBN: 978-0-85729-826-3

  • eBook Packages: MedicineMedicine (R0)

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