Imaging Strategies for Studying Mammalian Oogenesis
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Advances in biomedical imaging are now impacting the evaluation of gonads and gametes in experimental animal model systems and human clinical specimens. This chapter summarizes the rationale for both live cell and fixed sample imaging using light microscopy. Useful protocols, detailed methods, and tools for data extraction are covered for a variety of applications pertaining to the analysis of oocytes, embryos, and intact ovarian tissue.
KeywordsOogenesis Confocal microscopy Sample fixation Sample processing Vital staining Image processing Image archiving
The authors express their gratitude to the many colleagues and friends who have worked in the lab to advance the perspectives of oocyte biology that we enjoy today. Special thanks go to Lynda McGinnis and Britta Mattson who at different ends of the spectrum contributed to the development of MTSB-XF. Finally, this chapter would not have been possible without the funding from the NIH, ESHE Fund, The March of Dimes, and the Hall Family Foundation.
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