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Production of Hydrogen Peroxide During Hypoxia-Reoxygenation in Isolated Myocytes

  • Chapter
The Ischemic Heart

Part of the book series: Progress in Experimental Cardiology ((PREC,volume 1))

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Abstract

Active oxygen species, including hydrogen peroxide (H2O2), have been implicated in myocardial reperfusion injury. Recently, spin-trap agents and biochemical techniques applied to intact hearts have shown that H2O2 is generated by leukocytes, by endothelial cells, and by mitochondria in myocytes. In this study, we used electron microscopy and the cerium (Ce) method to histologically investigate H2O2 formation during hypoxia—reoxygenation and its toxic effects on myocardium. This Ce method involves the formation of an electron-dense precipitate when H2O2 reacts with cerium chloride (CeCl3). Single myocytes were obtained from rat hearts by the collagenase method. Isolated myocytes were reoxygenated for 15 minutes after 30 minutes of hypoxia. Digitonin and CeCl3, were added to make cell membranes permeable and to detect intracellular H2O2 by electron microscopy. In the control group, the ultrastructure was well preserved and no dense deposits were found in myocytes. However, in the hypoxia—reoxygenation group, precipitates, which were cerium—H2O2 reaction products, were found along swollen mitochondria. Moreover, in the hypoxia-reoxygenation group, cell viability was reduced to 72% of control. These results indicate that H2O2 is generated by mitochondria and that its relese into cytosol may lead to myocyte death during hypoxia—reperfusion.

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Authors and Affiliations

  1. Showa University School of Medicine, Japan

    Ryuji Ueda, Noburu Konno, Masaki Nakatani, Toshiki Iwata, Yoshihisa Arata, Katsumiti Iijima, Kazuaki Nishio, Eiichi Geshi, Toshikuni Yanagishita, Takashi Katagiri & Yasumitsu Nakai

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  1. Ryuji Ueda
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  2. Noburu Konno
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  3. Masaki Nakatani
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  4. Toshiki Iwata
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  5. Yoshihisa Arata
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  6. Katsumiti Iijima
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  7. Kazuaki Nishio
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  8. Eiichi Geshi
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  9. Toshikuni Yanagishita
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  10. Takashi Katagiri
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  11. Yasumitsu Nakai
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Editor information

Editors and Affiliations

  1. Department of Medicine Aoto Hospital, Jikei University School of Medicine, Tokyo, Japan

    Seibu Mochizuki M.D., Ph.D. (Professor and Chairman) (Professor and Chairman)

  2. Department of Internal Medicine Aoto Hospital, Jikei University School of Medicine, Tokyo, Japan

    Nobuakira Takeda M.D., Ph.D. (Associate Professor) (Associate Professor)

  3. Jikei University School of Medicine, Tokyo, Japan

    Makoto Nagano M.D., Ph.D. (Professor-Emeritus) (Professor-Emeritus)

  4. MRC Group in Experimental Cardiology Institute of Cardiovascular Sciences St. Boniface General Hospital Research Centre Faculty of Medicine, University of Manitoba, Winnipeg, Canada

    Naranjan S. Dhalla Ph.D., M.D. (Hon.), D.Sc. (Hon.) (Distinguished Professor and Director) (Distinguished Professor and Director)

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© 1998 Kluwer Academic Publishers

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Ueda, R. et al. (1998). Production of Hydrogen Peroxide During Hypoxia-Reoxygenation in Isolated Myocytes. In: Mochizuki, S., Takeda, N., Nagano, M., Dhalla, N.S. (eds) The Ischemic Heart. Progress in Experimental Cardiology, vol 1. Springer, Boston, MA. https://doi.org/10.1007/978-0-585-39844-0_7

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  • DOI: https://doi.org/10.1007/978-0-585-39844-0_7

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-0-7923-8105-1

  • Online ISBN: 978-0-585-39844-0

  • eBook Packages: Springer Book Archive

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