Intramyocardial Ejection of Basic Fibroblast Growth Factor Increased Regional Myocardial Blood Flow Andsalvaged Infarcted Myocardium in Dogs
We studied whether basic fibroblast growth factor (bFGF) might increase regional Myocardial blood flow (Qm) at the infarcted myocardium. In eight dogs, bFGF 300 μg was injected into the myocardium supplied by the left anterior descending coronary artery (LAD), and the artery was ligated. In 12 dogs, saline was injected (control group). Nonradioactive colored microspheres were used to determine Qm. The amount of viable myocardium and the extent of fibrosis in the infarcted area four weeks after occlusion were measured histologically. In the outer layer, the Qm values immediately after and four weeks after occlusion were 26 ± 2% and 70 ± 6%, respectively, in the control group, and 46 ± 5% and 121 ± 13%, respectively, in the bFGF group. The Qm at both times in the bFGF group was significantly higher than the corresponding control group values (p < 0.01). The Qm at four weeks in the inner and middle layers also significantly increased in the bFGF group. There was more viable myocardium (control vs. bFGF group: 41 ± 5% vs. 61 ± 7%, p < 0.05) and less fibrosis (3.1 ± 0.2 vs. 2.0 ± 0.4, p < 0.01) at the outer layer in the bFGF group. bFGF caused a marked increase of the Qm, an increase of viable myocardium, and a decrease of fibrosis at the infarcted myocardium. We conclude bFGF was effective in limiting infarct size in acute myocardial infarction.
KeywordsInfarct Size Left Anterior Descendng Myocardial Blood Flow Basic Fibroblast Growth Factor Viable Myocardium
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