Underperfusion Injury in Diabetic Rat Hearts: Effects of Norepinephrine and/or Insulin on Stiffness Increase and Abnormal Energy Metabolism

  • Makie Higuchi
  • Kanako Miyagi
  • Matao Sakanashi
Part of the Progress in Experimental Cardiology book series (PREC, volume 1)


Diabetic hearts were more susceptible than nondiabetic hearts to flow reduction and readily exhibited an increase in left ventricular diastolic stiffness in isolated rat hearts. Norepinephrine during underperfusion exacerbated the injury and improved the reperfusion injury, particularly in diabetic hearts, while the positive inotropic response was decreased by the progression of diabetes. The increase in stiffness correlated closely with ATP depletion and lactate accumulation in the subendocardium, which was metabolically more susceptible than the subepicardium. The correlation curves, however, were not coincidental: the critical ATP level was significantly higher in diabetic hearts. The markedly high glycogen content in diabetic hearts probably helps delay the start of underperfusion injury. The degree of injury depended on the degree and duration of underperfusion with norepinephrine and on the severity of diabetes. In vivo and ex vivo insulin prevented the injury.


Contractile Force Left Ventricular Pressure Heart Weight Diabetic Heart Coronary Pedusion Pressure 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Kluwer Academic Publishers 1998

Authors and Affiliations

  • Makie Higuchi
    • 1
  • Kanako Miyagi
    • 2
  • Matao Sakanashi
    • 2
  1. 1.Osaka University Medical SchoolJapan
  2. 2.University of the RyukyusJapan

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