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Thioctic Acid Prevents Oxidativedamage in Isolated Cardiac Myocytes

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The Ischemic Heart

Part of the book series: Progress in Experimental Cardiology ((PREC,volume 1))

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Abstract

Thioctic acid and its reduced form, dihydrolipoic acid, may protect myocardium against ischemia-reperfusion damage. It is unknown whether or not thioctic acid exerts its protective action by any direct effect on heart muscle cells. The in vitro study reported here aimed to investigate the effect of thioctic acid against oxidative damage in singlet oxygen-challenged isolated cardiac myocytes. Cardiac myocytes were isolated from adult rat hearts by collagenase perfusion. Singlet oxygen was generated by photoexcitation of rose bengal (0.1 μM). Exposure to singlet oxygen induced irreversible hypercongracture in 70% to 80% of rod-shaped cardiomyocytes accompanied by a significant increase in the production of thiobarbituric acid reactive substances as an indicator for lipid peroxidation. Pretreatment of the cardiomyocytes with thioctic acid (1 mM) significantly increased the number of surviving cardiomyocytes afier exposure to singlet oxygen to 52.6% ± 9.6% vs. 22.2% ± 6.3% (p < 0.5) and reduced the amount of thiobarbituric acid reactive substances to 385 ± 42 vs. 635 ± 90 nmol/g protein (p < 0.5). The data suggest a cardoprotective effect of thioctic acid, which is possibly due to its antioxidative action.

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Author information

Authors and Affiliations

  1. Kardiologie, Medizinische Klinik IV, USA

    Hans-Georg Olbrich, P. Grabisch, T. Dickfeld & I. Henke

  2. Johann Wolfgang Goethe-Universität, USA

    E. Mutschler

Authors
  1. Hans-Georg Olbrich
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  2. P. Grabisch
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  3. T. Dickfeld
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  4. I. Henke
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  5. E. Mutschler
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Editor information

Editors and Affiliations

  1. Department of Medicine Aoto Hospital, Jikei University School of Medicine, Tokyo, Japan

    Seibu Mochizuki M.D., Ph.D. (Professor and Chairman) (Professor and Chairman)

  2. Department of Internal Medicine Aoto Hospital, Jikei University School of Medicine, Tokyo, Japan

    Nobuakira Takeda M.D., Ph.D. (Associate Professor) (Associate Professor)

  3. Jikei University School of Medicine, Tokyo, Japan

    Makoto Nagano M.D., Ph.D. (Professor-Emeritus) (Professor-Emeritus)

  4. MRC Group in Experimental Cardiology Institute of Cardiovascular Sciences St. Boniface General Hospital Research Centre Faculty of Medicine, University of Manitoba, Winnipeg, Canada

    Naranjan S. Dhalla Ph.D., M.D. (Hon.), D.Sc. (Hon.) (Distinguished Professor and Director) (Distinguished Professor and Director)

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© 1998 Kluwer Academic Publishers

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Olbrich, HG., Grabisch, P., Dickfeld, T., Henke, I., Mutschler, E. (1998). Thioctic Acid Prevents Oxidativedamage in Isolated Cardiac Myocytes. In: Mochizuki, S., Takeda, N., Nagano, M., Dhalla, N.S. (eds) The Ischemic Heart. Progress in Experimental Cardiology, vol 1. Springer, Boston, MA. https://doi.org/10.1007/978-0-585-39844-0_30

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  • DOI: https://doi.org/10.1007/978-0-585-39844-0_30

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-0-7923-8105-1

  • Online ISBN: 978-0-585-39844-0

  • eBook Packages: Springer Book Archive

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