Skip to main content
SpringerLink
Log in
Book cover

The Ischemic Heart pp 337–349Cite as

Hypoxic Preconditioning of Isolated Cardiomyocytes of Adult Rat

  • Chapter

  • 1695 Accesses

Part of the book series: Progress in Experimental Cardiology ((PREC,volume 1))

Abstract

The present study was undertaken to examine whether or not cytoprotective effects of hypoxic preconditioning were detectable in isolated, quiescent cardiomyocytes of adult rats. The cardiomyocytes were incubated for 120 minutes under hypoxic condtions (sustained hypoxia), followed by 15-minute reoxygenation. Sustained hypoxia decreased the number of viable cells (from 99% to 70% of the initial cell), which consisted of rod- and square-shaped cardiomyocytes. It also decreased the number of rod-shaped cardiomyocytes (from 90% to 40% of the initial cell) and simultaneously increased the number of square-shaped cells (from 10% to 30% of the initial cell). Fifteen-minute reoxygenation resulted in a further decrease in the numbers of viable cells (less than 50% of the initial cell) and square-shaped cells (10% of the initial cell), whereas it did not change the number of rod-shaped cells. Hypoxia-reoxygenation also induced a release of purine nucleosides and bases (ATP metabolites) into the incubation medium. When the cardomyocytes were subjected to 20 minutes of hypoxic incubation, followed by 30 minutes of normoxic incubation (hypoxic preconditioning), sustained hypoxia-induced decreases in the numbers of viable cells and rod-shaped cells were attenuated (80% and 60% of the initial cell, respectively). The intervention also attenuated sustained hypoxia-induced increase in the number of square-shaped cells (18% of the initial cell). The number of rod-shaped cells subjected to hypoxic preconditioning at the end of 15-minute reoxygenation was similar to that at the end of sustained hypoxia, whereas the number of square-shaped cells decreased to 10% of the initial cells, which was similar to that of square-shaped cells without hypoxic preconditioning. The intervention also suppressed the release of ATP metabolites during hypoxia-reoxygenation.

This is a preview of subscription content, log in via an institution.

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   129.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   299.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Murry CE, Jennings RB, Reimer KA. 1986. Preconditioning with ischemia: a delay of lethal cell injury in ischemic myocardium. Circulation 74:1124–1136.

    PubMed  CAS  Google Scholar 

  2. Shiki K, Hearse DJ. 1987. Preconditioning of ischemic myocardium: reperfusion induced arrhythmias. Am J Physiol 253:H1470–H1476.

    PubMed  CAS  Google Scholar 

  3. Murry CE, Richard VJ, Reimer KA, Jennings RB. 1990. Ischemic preconditioning slows energy metabolism and delays ultrastructural damage during sustained ischemic episode. Circ Res 66:913–931.

    PubMed  CAS  Google Scholar 

  4. Hagar JM, Hale SL, Kloner RA. 1991. Effect of preconditioning ischemia on reperfusion arrhythmias after coronary artery occlusion and reperfusion in the rat. Circ Res 68:61–68.

    PubMed  CAS  Google Scholar 

  5. Liu GS, Richards SC, Olsson RA, Mullane K, Walsh RS, Downey JM. 1994. Evidence that the adenosine A3 receptor may mediate the protection afforded by preconditioning in the isolated rabbit heart. Cardiovasc Res 28:1057–1061.

    PubMed  CAS  Google Scholar 

  6. Armstrong S, Ganote CE. 1994. Adenosine receptor specificity in preconditioning of isolated rabbit cardiomyocytes: evidence of A3 receptor involovement. Cardiovasc Res 28:1049–1056.

    PubMed  CAS  Google Scholar 

  7. Armstrong S, Downey JM, Ganote CE. 1994. Preconditioning of isolated rabbit cardiomyocytes: induction by metabolic stress and blockade by the adenosine antagonist SPT and calphostin C, a protein kinase inhibitor. Cardiovasc Res 28:72–77.

    PubMed  CAS  Google Scholar 

  8. Gottlieb RA, Gruol DL, Zhu JY, Engler U. 1996. Preconditioning in rabbit cardiomyocytes. Role of pH, vacuolar proton ATPase, and apoptosis. J Clin Invest 97:2391–2398.

    Article  PubMed  CAS  Google Scholar 

  9. Zhou X, Zhai X, Ashraf M. 1996. Direct evidence that initial oxidative stress triggered by preconditioning contributes to second window of protection by endogenous antioxidant enzyme in myocytes. Circulation 93:1177–1184.

    PubMed  CAS  Google Scholar 

  10. Hayashi M, Nasa Y, Tanonaka K, Sasaki H, Miyake R, Hayashi J, Takeo S. 1995. The effects of long-term treatment with eicosapentaenoic acid and docosahexaenoic acid on hypoxia/reoxygenation injury of isolated cardiac cells in adult rats. J Mol Cell Cardiol 27:2031–2041.

    Article  PubMed  CAS  Google Scholar 

  11. Liu J, Tanonaka K, Sanbe A, Yamamoto K, Takeo S. 1993. Beneficial effects of quinidine on post-ischemic contractile failure of isolated rat hearts. J Mol Cell Cardiol 25:1249–1263.

    Article  PubMed  CAS  Google Scholar 

  12. Takeo S, Tanonaka K, Miyake K, Fukumoto T. 1988. Role of ATP metabolites in induction of incomplete recovery of cardiac contractile force after hypoxia. Can J Cardiol 4:193–200.

    PubMed  CAS  Google Scholar 

  13. Takeo S, Tanonaka K, Miyake K, Fukumoto T. 1988. Adenine nucleotide metabolites are beneficial for recovery of cardiac contractile force after hypoxia. J Mol Cell Cardiol 20:187–199.

    Article  PubMed  CAS  Google Scholar 

  14. Tanonaka K, Matsumoto M, Minematsu R, Miyake K, Murai R, Takeo S. 1989. Beneficial effect of amosulalol and phentolamine on post-hypoxic recovery of contractile force and energy metabolism in rabbit hearts. Br J Pharmacol 97:513–523.

    PubMed  CAS  Google Scholar 

  15. Liu J, Tanonaka K, Ohtsuka Y, Sakai Y, Takeo S. 1993. Improvement of ischemia/reperfusion-induced contractile dysfunction of perfused hearts by class Ic antiarrhythmic agents. J Pharmacol Exp Ther 266:1247–1254.

    PubMed  CAS  Google Scholar 

  16. Takeo S, Tanonaka K, Tazuma Y, Fukao N, Yoshikawa C, Fukumoto T, Tanaka T. 1988. Diltiazem and verapamil reduce the loss of adenine nucleotide metabolism from hypoxic hearts. J Mol Cell Cardiol 20:187–199.

    Article  PubMed  CAS  Google Scholar 

  17. Tanonaka K, Maruyama Y, Takeo S. 1991. Beraprost, a prostacyclin mimetic agent, is beneficial for post-hypoxic recovery of cardiac function and metabolites in isolated rabbit hearts. Br J Pharmacol 104:779–786.

    PubMed  CAS  Google Scholar 

  18. Schrader J, Haddy F, Gerlach E. 1977. Release of adenosine, inosine and hypoxanthine from the isolated guinea pig heart during hypoxia. Pflugers Arch 369:1–6.

    Article  PubMed  CAS  Google Scholar 

  19. Vary TC, Angelakos ET, Schaffer SW. 1979. Relationship between adenine nucleotide metabolism and irreversible ischemic tissue damage in isolated perfused rat heart. Circ Res 45:218–225.

    PubMed  CAS  Google Scholar 

  20. Takeo S, Sakanashi M. 1983. Possible mechanisms for reoxygenation-induced recovery of myocardial high-energy phosphates after hypoxia. J Mol Cell Cardiol 15:577–594.

    Article  PubMed  CAS  Google Scholar 

  21. Takeo S, Tanonaka K, Shimizu K, Hirai K, Miyake K, Minematsu R. 1989. Beneficial effects of lidocaine and disopyramide on oxygen-deficiency-induced contractile failure and metabolic disturbance in isolated rabbit hearts. J Pharmacol Exp Ther 248:306–314.

    PubMed  CAS  Google Scholar 

  22. Takeo S, Yamada H, Tanonaka K, Hayashi M, Sunagawa N. 1990. Possible involvement of membrane-stabilizing action in beneficial effect of beta adrenoceptor blocking agents on hypoxic and posthypoxic myocardium. J Pharmacol Exp Ther 254:847–856.

    PubMed  CAS  Google Scholar 

  23. Fujioka H, Yoshihara S, Tanaka T, Fukumoto T, Kuroiwa A, Tanonaka K, Hayashi M, Takeo S. 1991. Enhancement of post-hypoxic contractile and metabolic recovery of perfused rat hearts by dlpropranolol: possible involvement of non-beta-receptor mediated activity. J Mol Cell Cardiol 23:949–962.

    Article  PubMed  CAS  Google Scholar 

  24. Armstrong S, Ganote CE. 1994. Preconditioning of isolated rabbit cardiomyocytes: effects of glycolytic blockade, phorbol esters, and ischaemia. Cardiovasc Res 28:1700–1706.

    PubMed  CAS  Google Scholar 

  25. Speechly-Dick ME, Mocanu MM, Yellon DM. 1994. Protein kinase C: its role in ischemic preconditioning in the rat. Circ Res 75:586–590.

    PubMed  CAS  Google Scholar 

  26. Ytrehus K, Liu Y, Downey JM. 1994. Preconditioning protects ischemic rabbit heart by protein kinase C activation. Am J Physiol 266:H1145–H1152.

    PubMed  CAS  Google Scholar 

  27. Li Y, Kloner RA. 1995. Does protein kinase C play a role in ischemic preconditioning in rat hearts? Am J Physiol 268:H426–H431.

    PubMed  CAS  Google Scholar 

  28. Liu Y, Tsuchida A, Cohen MV, Downey JM. 1995. Pretreatment with angiotensin II activates protein kinase C and limits myocardial infarction in isolated rabbit hearts. J Mol Cell Cardiol 27:883–892.

    Article  PubMed  CAS  Google Scholar 

  29. Mitchell MB, Meng X, Ao L, Brown JM, Harken AH, Banerjee A. 1995. Preconditioning of isolated rat heart is mediated by protein kinase C. Circ Res 76:73–81.

    PubMed  CAS  Google Scholar 

  30. Goto M, Liu Y, Yang X-M, Ardell JL, Cohen MV, Downey JM. 1995. Role of bradykinin in protection of ischemic preconditioning in rabbit hearts. Circ Res 77:611–621.

    PubMed  CAS  Google Scholar 

  31. Armstrong S, Ganote CE. 1995. In vitro ischaemic preconditioning of isolated rabbit cardiomyocytes: effects of selective adenosine receptor blockade and calphostin C. Cardiovasc Res 29:647–652.

    Article  PubMed  CAS  Google Scholar 

  32. Armstrong SC, Hoover DB, Delacey MH, Ganote CE. 1996. Translocation of PKC, protein phosphatase inhibition and preconditioning of rabbit cardiomyocytes. J Mol Cell Cardiol 28:1479–1492.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Tokyo University of Pharmacy and Life Science, Japan

    Michiko Nojiri, Kouchi Tanonaka, Ken-Ichi Yabe & Satoshi Takeo

Authors
  1. Michiko Nojiri
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kouchi Tanonaka
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ken-Ichi Yabe
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Satoshi Takeo
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Department of Medicine Aoto Hospital, Jikei University School of Medicine, Tokyo, Japan

    Seibu Mochizuki M.D., Ph.D. (Professor and Chairman) (Professor and Chairman)

  2. Department of Internal Medicine Aoto Hospital, Jikei University School of Medicine, Tokyo, Japan

    Nobuakira Takeda M.D., Ph.D. (Associate Professor) (Associate Professor)

  3. Jikei University School of Medicine, Tokyo, Japan

    Makoto Nagano M.D., Ph.D. (Professor-Emeritus) (Professor-Emeritus)

  4. MRC Group in Experimental Cardiology Institute of Cardiovascular Sciences St. Boniface General Hospital Research Centre Faculty of Medicine, University of Manitoba, Winnipeg, Canada

    Naranjan S. Dhalla Ph.D., M.D. (Hon.), D.Sc. (Hon.) (Distinguished Professor and Director) (Distinguished Professor and Director)

Rights and permissions

Reprints and permissions

Copyright information

© 1998 Kluwer Academic Publishers

About this chapter

Cite this chapter

Nojiri, M., Tanonaka, K., Yabe, KI., Takeo, S. (1998). Hypoxic Preconditioning of Isolated Cardiomyocytes of Adult Rat. In: Mochizuki, S., Takeda, N., Nagano, M., Dhalla, N.S. (eds) The Ischemic Heart. Progress in Experimental Cardiology, vol 1. Springer, Boston, MA. https://doi.org/10.1007/978-0-585-39844-0_25

Download citation

  • DOI: https://doi.org/10.1007/978-0-585-39844-0_25

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-0-7923-8105-1

  • Online ISBN: 978-0-585-39844-0

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation