Regulation of [Na+i and [Ca2+]i during Myocardial Ischemia and Reperfusion in a Single-Cell Model

  • Hideharu Hayashi
  • Hiroshi Satoh
  • Hideki Katoh
  • Takuro Nakamura
  • Shiho Sugiyama
  • Hajime Terada
Part of the Progress in Experimental Cardiology book series (PREC, volume 1)


To study the regulation of [Na+]i and [Ca2+]i during metabolic inhibition (MI) by the perfusion of 3.3 mM amytal and 5 μM CCCP, [Na+]i and [Ca2+], were measured simultaneously using guinea pig ventricular myocytes that were dual-loaded with SBFI/M and fluo-3/AM. It was suggested that 1) [Na+]i increased during MI by both the activated Na+ influx via Na+-H+ exchange and the suppressed Na+ extrusion via the Na+-K+ pump, 2) Na+-Ca2+ exchange was inhibited during MI, causing the dissociation between [Na+]i and [Ca2+]i, 3) Na+-Ca2+ exchange could be reactivated by energy repletion, resulting in an increase of [Ca2+]i and 4) cell contracture during MI was related to rigor due to energy depletion, while cell contracture after energy repletion was likely to be related to Ca2+ overload. We also investigated the regulation of [Na+]i, [Ca2+]i, and pHi during simulated ischemia (MI with extracellular acidosis) and reperfusion. Na+-H+ exchange was active during simulated ischemia. After reperfusion, Na+-H+ exchange was activated further as pHi was recovered, resulting in an additional [Na+]i elevation.


Metabolic Inhibition Energy Depletion Cell Contracture Intracellular Acidosis Simulated Ischemia 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Kluwer Academic Publishers 1998

Authors and Affiliations

  • Hideharu Hayashi
    • 1
  • Hiroshi Satoh
    • 1
  • Hideki Katoh
    • 1
  • Takuro Nakamura
    • 1
  • Shiho Sugiyama
    • 1
  • Hajime Terada
    • 1
  1. 1.Hamamatsu University School of MedicineJapan

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