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Intracellular Calcium Regulation in Cardiac Cells during Acidosis

  • Cesare M. N. Terracciano
  • Kenneth T. Macleod
Part of the Progress in Experimental Cardiology book series (PREC, volume 1)

Abstract

The effects of intracellular acidosis on Ca2+ regulation mechanism in enzymatically isolated guinea pig ventricular myocytes were studied. Comparable degrees of acidosis were obtained by NH4Cl removal or application of lactate. Intracellular pH and [Ca2+] were monitored using the fluorescent indicators 2′-7′-bis-(carboxyethyl)-5(6)-carboxyfluorescein (BCECF) and indo-1. Release of Ca2+ from the sarcoplasmic reticulum was produced by rapid cooling or application of caffeine. Using caffeine, we have shown that acidosis affects Na+-Ca2+ exchange-mediated Ca2+ extrusion. This effect was not observed when the increase of intracellular Na+ during acidosis was prevented by blocking the Na+-H+ exchange. Using Na+-free/Ca2+-free solution to study the activity of the SR Ca2+ ATPase on cytoplasmic Ca2+, Ca2+ extrusion was slower in the presence of lactate but was unchanged after removal of NH4Cl. Using paired rapid cooling contractures, the relative contributions of the cellular mechanisms to Ca2+ extrusion were investigated. In acidosis produced by application of lactate and acetate, the relative role of the SR Ca2+ uptake was reduced, probably in favor of Na+-Ca2+ exchange.

Keywords

Sarcoplasmic Reticulum Ventricular Myocytes Intracellular Acidosis Normal Tyrode Normal Tyrode Solution 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Kluwer Academic Publishers 1998

Authors and Affiliations

  • Cesare M. N. Terracciano
    • 1
  • Kenneth T. Macleod
    • 1
  1. 1.Imperial College School of Medicine at National Heart & Lung InstituteUK

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