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Culture of cells of the osteoblast lineage

  • Francis J. Hughes
  • Jane E. Aubin

Abstract

Osteoblastic cell cultures have been utilized to study a wide range of issues in bone biology, including the regulation of the differentiation and metabolic activity of bone cells. These studies have included, for example: the investigation of the action of hormones, cytokines and other signalling molecules on bone cells; the molecular mechanisms of action of such factors; studies of the osteoblast cell lineage and differentiation-dependent changes in phenotype; the synthesis of matrix proteins and other secreted molecules; and studies of the interaction of bone cells with biomaterials. Some obvious advantages of such methods over experiments conducted in vivo include the ability to control more fully the cells being assessed and their environment, the relative ease of sampling and analysing changes in the parameters under investigation, and the ability to avoid or reduce animal experimentation.

Keywords

Osteoblastic Cell Bone Marrow Stromal Cell Mineral Research Osteoblast Lineage Bone Sialoprotein 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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© Chapman and Hall Ltd 1998

Authors and Affiliations

  • Francis J. Hughes
  • Jane E. Aubin

There are no affiliations available

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