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Development of an IRF-1 Based Proliferation Control System

  • P. P. Mueller
  • A. V. Carvalhal
  • J. L. Moreira
  • C. Geserick
  • K. Schroeder
  • M. J. T. Carrondo
  • H. Hauser
Part of the Cell Engineering book series (CEEN, volume 1)

Abstract

Mammalian cell cultures are the preferred production system for secreted pharmaceutical proteins (Hauser, 1997). Life of a mammal begins with fertilization of the oocyte that divides and proliferates until the animal reaches its mature size. Further cell growth is tightly controlled. Proliferation and cell death are balanced to keep the total cell mass essentially constant, while the synthesis and secretion of cellular products continues. Despite rapid progress in the understanding of growth regulatory mechanisms, tumorigenic growth due the loss of proliferation control of a single body cell initially is still a leading cause of disease and death.

Keywords

Cell Growth Inhibition Interferon Regulatory Factor Internal Ribosomal Entry Site Producer Cell Line Proliferation Control 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Kluwer Academic Publishers 1999

Authors and Affiliations

  • P. P. Mueller
    • 1
  • A. V. Carvalhal
    • 2
  • J. L. Moreira
    • 2
  • C. Geserick
    • 1
    • 3
  • K. Schroeder
    • 1
  • M. J. T. Carrondo
    • 2
  • H. Hauser
    • 1
  1. 1.Department of Gene Regulation and DifferentiationGBF - National Research Center for BiotechnologyBraunschweigGermany
  2. 2.ITQB/IBET- Instituto de Tecnologia Quimica e Biologica/Instituto de Biologica Experimental e TecnologiaOeirasPortugal
  3. 3.Novo NordiskGentofteDenmark

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