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Cell Engineering pp 162–185Cite as

Inhibition of Apoptosis In Mammalian Cell Culture

The Biotechnological Relevance of Limiting Cell Death

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Part of the book series: Cell Engineering ((CEEN,volume 1))

Abstract

When J.F. Enders first used primate cells to produce poliomyelitis virus in 1949, a multi-billion dollar industry was born. The realization that viruses could be attenuated in vitro for use as vaccines initiated attempts to develop large scale cultures of mammalian cells. In the 1970’s, two discoveries led to the expanded use of such cultures: (1) the advent of recombinant DNA technology meant that cell lines could be engineered to overexpress heterologous genes, and (2) the development of hybridoma cell lines provided a system capable of continuously secreting antibodies (Kohler and Milstein, 1975). Today, dozens of products including virus vaccines, antibodies, interferons, immunoregulators, hormones, and growth factors are manufactured by mammalian cells in culture; this number promises only to increase as our understanding of cellular processes grows.

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  1. Department of Chemical Engineering, The Johns Hopkins University, 3400 North Charles Street, Baltimore, Maryland, 21218, USA

    A. J. Mastrangelo

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    Mohamed Al-Rubeai

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Mastrangelo, A.J. (1999). Inhibition of Apoptosis In Mammalian Cell Culture. In: Al-Rubeai, M. (eds) Cell Engineering. Cell Engineering, vol 1. Springer, Dordrecht. https://doi.org/10.1007/978-0-585-37971-5_6

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