Abstract
This work was aimed at developing an efficient stirred-tank photobioreactor for the large- scale cultivation of photosynthetic cells. A quantitative method of evaluating light conditions inside photobioreactors was first investigated and a light-supply coefficient, which is a product of a light-distribution coefficient and light energy supplied per unit volume, was proposed as an engineering parameter for design and scale-up of photobioreactors. Using this parameter, a method of designing and scaling-up internally illuminated photobioreactors was proposed. A photobioreactor was considered as consisting of units, and an optimum unit size was defined as a reactor volume that is optimally illuminated by a centrally located single light source. A large-scale photobioreactor with the optimum light-supply coefficient can thus be constructed by determining the optimum unit size for the target process and then increasing the number of units in three dimensions. Based on this concept, an optimum unit was constructed and then scaled-up to 20.0 L while maintaining a constant light-supply coefficient. With a 20-W fluorescent lamp, the unit size (diameter) that gave the optimum light-supply coefficient for the cultivation of Chlorella was 0.075 m. Carbon dioxide fixation by Chlorella pyrenoidosa and α-tocopherol production by Euglena gracilis cells in the 20.0-L photobioreactor were the same as those of the single unit.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Camacho-Rubio, F., Padial-Vico, A., and Martinez-Sancho, M.E., 1985, The effect of the mean intensity of light on the cultivation of Chlorella pyrenoidosa, Inter. Chem. Eng., 25:283–288.
Hirata, S., Hayashitani, M., Taya, M., and Tone, S., 1996, Carbon dioxide fixation in batch culture of Chlorella sp. using a photobioreactor with a sunlight collection device, J. Ferment. Bioeng., 81:470–472.
Hoshino, K., Hamochi, M., Mitsuhashi, S., and Tanishita, K., 1991, Measurements of oxygen production rate in flowing Spirulina suspension, Appl. Microbiol. Biotechnol., 35:89–93.
Javanmardian, M., and Palsson, B.O., 1991, High-density photoautotrophic algal cultures: design, construction, and operation of a novel photobioreactor system, Biotechnol. Bioeng., 38:1182–1189.
Ju, L.K., and Chase, G.G., 1992, Improved scale-up strategies of bioreactors, Bioproc. Eng., 8:49–53.
Märkl, H., 1977, CO2 transport and photosynthetic productivity of a continuous culture of algae, Biotechnol. Bioeng., 19:1851–1862.
Mori, K., 1985, Photoautotrophic bioreactor using solar rays condensed by fresnel lenses, Biotechnol. Bioeng. Symp., 15:331–345.
Miyamoto, K., Wable, O., and Benemann, J.R., 1988, Vertical tubular reactor for microalgae cultivation, Biotechnol. Lett., 10:703–708.
Ogawa, T., Kozawa, H., and Terui, G., 1971, Studies on the growth of Spirulina platensis (II) growth kinetics of an autotrophic culture, J. Ferment. Technol., 50:143–149.
Ogbonna, J.C., Yada, H., and Tanaka, H., (1995a), Effect of cell movement by random mixing between the surface and bottom of photobioreactors on algal productivity, J. Ferment. Bioeng., 79:152–157.
Ogbonna, J.C., Yada, H., and Tanaka, H., 1995b, Kinetic study of light-limited batch cultivation of photosynthetic cells, J. Ferment. Bioeng., 80:259–264.
Ogbonna, J.C., Yada, H., and Tanaka, H., 1995c, Light supply coefficient—a new engineering parameter for photobioreactor design, J. Ferment. Bioeng., 80, 369–376.
Ogbonna, J.C., Yada, H., Masui, H, and Tanaka, H., 1996, A novel internally illuminated stirred tank photobioreactor for large scale cultivation of photosynthetic cells, J. Ferment. Bioeng., 82:61–67.
Ogbonna, J.C., and Tanaka, H., 1996, Night biomass loss and changes in biochemical composition of cells during light/dark cyclic culture of Chlorella pyrenoidosa, J. Ferment. Bioeng., 82:558–564.
Pirt, J.S., Lee, Y.K., Walach, M.R., Pirt, M.W., Balyuzi, H.H.M., and Bazin, M.J., 1983, A tubular bioreactor for photosynthetic production of biomass from carbon dioxide: design and performance, J. Chem. Tech. Biotechnol., 33B:35–58.
Richmond, A., Boussiba, S., Vonshak, V., and Kopel, R., 1993, A new tubular reactor for mass production of microalgae outdoors, J. Appl. Phycol., 5:327–332.
Shigeoka, S., Onishi, T., Nakano, Y., and Kitaoka, S., 1986, The contents and subcellular distribution of tocopherols in Euglena gracilis, Agric. Biol. Chem., 50:1063–1065.
Sumino, Y., Sonoi, K., and Doi, M., 1993, Scale-up of purine nucleoside fermentation from a shaking flask to a stirred-tank fermentor, Appl. Microbiol. Biotechnol., 38:581–585.
Tanaka, H., Ishikawa, H., Nobayashi, H., and Takagi, Y., 1991, A new scale-up method based on the effect of ventilation on aerated fermentation systems.
Terry, K.L., and Raymond, L.P., 1985, System design for the autotrophic production of microalgae, Enzyme Microb. Technol., 7:474–487.
Torzillo, G., Carlozzi, P., Pushparaj, B, Montaini, E., and Materassi, R., 1993, A two-plane photobioreactor for outdoor culture of Spirulina, Biotechnol. Bioeng., 42:891–898.
Tredici, M.R., Carlozzi, P. Zittelli, G.C., and Materassi, R., 1991, A vertical alveolar panel (VAP) for outdoor mass cultivation of microalgae and cyanobacteria, Biores. Technol., 38:153–159.
Tredici, M.R., and Materassi, R., 1992, From open ponds to vertical alveolar panels: the Italian experience in the development of reactors for the mass cultivation of phototrophic microorganisms, J. Appl. Phycol., 4:221–231.
Watanabe, Y., Noüe, J., and Hall, D.O., 1995, Photosynthetic performance of a helical tubular photobioreactor incorporating the cyanobacterium Spirulina platensis, Biotechnol. Bioeng., 47:261–269.
Yakunin, A.F., Tsygankov, A.A., Gogotov, I.N., and L’vov, N.P., 1986, Dependence of the nitrogenase and nitrate reductase activities on the molybdenum concentration in the medium and assimilation of nitrate in cells of Rhodopseudomonas capsulata, Mikrobiologiya, 55:564–569.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1998 Plenum Press, New York
About this chapter
Cite this chapter
Ogbonna, J.C., Soejima, T., Tanaka, H. (1998). Development of Efficient Large-Scale Photobioreactors. In: Zaborsky, O.R., Benemann, J.R., Matsunaga, T., Miyake, J., San Pietro, A. (eds) BioHydrogen. Springer, Boston, MA. https://doi.org/10.1007/978-0-585-35132-2_41
Download citation
DOI: https://doi.org/10.1007/978-0-585-35132-2_41
Publisher Name: Springer, Boston, MA
Print ISBN: 978-0-306-46057-9
Online ISBN: 978-0-585-35132-2
eBook Packages: Springer Book Archive