Abstract
The cyanobacterium Spirulina platensis accumulates glycogen photoautotrophically in a nitrogen-deficient medium. Under anaerobic conditions in the dark, the glycogen degrades into organic compounds. As molecular hydrogen also evolves in this process, hydrogenase participation is suggested in this metabolism. We investigated the several conditions necessary for the evolution of hydrogen and production of organic compounds. The effects of cell concentration, initial pH, and concentration of the buffer were determined.
These fermentation products were then converted into molecular hydrogen by using the photosynthetic bacterium Rhodobacter sphaeroides RV with light energy. The composition of the evolved gas was mainly hydrogen and carbon dioxide. This photosynthetic bacterial production of hydrogen was caused by a nitrogenase-dependent mechanism. Combining this system with photosynthesis of cyanobacteria resulted in the production of hydrogen by splitting water.
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References
Asada, Y., and Kawamura, S., 1984, Hydrogen evolution by Microcystis aeruginosa in darkness, Agric. Biol. Chem., 48:2595–2596.
Asada, Y., and Kawamura, S., 1986, Screening for cyanobacteria that evolve molecular hydrogen under dark and anaerobic conditions, J. Ferment. Technol., 64:553–556.
Ernst, A., Kirschenlohr, H., Diez, J., and Boeger, P., 1984, Glycogen content and nitrogenase activity in Anabaena variabilis, Arch. Microbial., 140:120–125.
Miura, Y., Saitoh, C., Matsuoka, S., and Miyamoto, K., 1992, Stably sustained hydrogen production with high molar yield through a combination of a marine green alga and a photosynthetic bacterium, Biosci. Biotech. Biochem., 56:751–754.
Miyake, J., Mao, X.Y., and Kawamura, S., 1984, Photoproduction of hydrogen from glucose by a co-culture of a photosynthetic bacterium and Clostridium butyricum, J. Ferment. Technol., 62:531–535.
Miyamoto, K., 1993, Hydrogen production by photosynthetic bacteria and microalgae, in Recombinant Microbes for Industrial and Agricultural Applications, Murooka, Y., and Imanaka, T. (eds.), Marcel Dekker, New York, pp. 771–786.
Ogawa, T., and Terui, G., 1970, Studies on the growth of Spirulina platensis, I. on the pure culture of Spirulina platensis. J. Ferment. Technol., 48:361–367.
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© 1998 Plenum Press, New York
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Aoyama, K., Uemura, I., Miyake, J., Asada, Y. (1998). Photosynthetic Bacterial Hydrogen Production with Fermentation Products of Cyanobacterium Spirulina platensis . In: Zaborsky, O.R., Benemann, J.R., Matsunaga, T., Miyake, J., San Pietro, A. (eds) BioHydrogen. Springer, Boston, MA. https://doi.org/10.1007/978-0-585-35132-2_38
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DOI: https://doi.org/10.1007/978-0-585-35132-2_38
Publisher Name: Springer, Boston, MA
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