Abstract
A shuttle vector (pKE4-9) system for a cyanobacterium, Synechococcus sp.PCC7942 and Escherichia coli, was examined for expression of clostridial hydrogenase. pKE4-9 carries the chloramphenicol-resistant (cat) gene and a strong promoter we have isolated from PCC7942. The clostridial hydrogenase gene was cloned upstream of the cat gene in pKE4-9, which was transformed into PCC7942. Although the cat protein was expressed, the clostridial hydrogenase was not detected by Western blot analysis. The hydrogenase mRNA was detected in PCC7942; it was co-transcribed with cat. The problems in the expression of foreign proteins in cyanobacteria are discussed.
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© 1998 Plenum Press, New York
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Koike, Y. et al. (1998). Attempt at Heterologous Expression of Clostridial Hydrogenase in Cyanobacteria. In: Zaborsky, O.R., Benemann, J.R., Matsunaga, T., Miyake, J., San Pietro, A. (eds) BioHydrogen. Springer, Boston, MA. https://doi.org/10.1007/978-0-585-35132-2_14
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DOI: https://doi.org/10.1007/978-0-585-35132-2_14
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