Abstract
A method has been proposed for the enhancement of bacterial light-dependent hydrogen production by altering the LH1/LH2 ratio of Rhodobacter sphaeroides RV. A model mutant strain, P3, was obtained by UV irradiation from the wild strain (RV). The amount of bacteriochlorophyll in LH1 was reduced to 35% and that in LH2 was enhanced to 140% in P3 compared to RV. P3 showed 1.4 times higher hydrogen production rates than RV at 850 nm (an absorption maxima for LH2). At 875 nm (absorption maximum for LH1), P3 showed a similar hydrogen production rate to RV, despite the decreased level of LH1. P3 was restored to the wild-type LH1/LH2 ratio by genetic transformation with a plasmid carrying genes encoding LH1 pigment-binding proteins. The hydrogen production of the transformed P3 was also restored to that of the wild type. These results suggest that alteration of the LH1/LH2 ratio can enhance light-dependent hydrogen production.
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© 1998 Plenum Press, New York
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Miyake, M. et al. (1998). Improvement of Bacterial Light-Dependent Hydrogen Production by Altering the Photosynthetic Pigment Ratio. In: Zaborsky, O.R., Benemann, J.R., Matsunaga, T., Miyake, J., San Pietro, A. (eds) BioHydrogen. Springer, Boston, MA. https://doi.org/10.1007/978-0-585-35132-2_10
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DOI: https://doi.org/10.1007/978-0-585-35132-2_10
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