Organization of The Interphotoreceptor Matrix

Binding of SPACR to Hyaluronan
  • Joe G. Hollyfield
  • Mary E. Rayborn
  • Shreeta Acharya


The distribution of hyaluronan (HA) in the interphotoreceptor matrix (IPM) was evaluated in human tissues using a specific probe for HA (bHABC, biotinylated hyaluronan binding complex). In tissue sections, bHABC heavily decorates HA in the matrix surrounding rods and shows lighter binding to the cone associated matrix. Both HA and the glycoprotein SPACR are present in insoluble IPM isolates removed from unfixed retinas with distilled water treatment. To determine whether SPACR associates with HA, precipitation experiments were performed using the detergent cetylpyridinium chloride (CPC), in conjunction with hyaluronidase digestions. SPACR is present in the CPC pellet when an undigested IPM sample is precipitated, but is not present in the CPC pellet when the IPM is first digested with Streptomyces hyaluronidase. These results indicate that SPACR binds to HA, and implicates HA as the primary scaffold onto which IPM molecules form associative interactions in the IPM.


Hyaluronic Acid Human Retina Associative Interaction Hyaluronan Binding Interphotoreceptor Matrix 
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Copyright information

© Kluwer Academic / Plenum Publishers 1999

Authors and Affiliations

  • Joe G. Hollyfield
    • 1
  • Mary E. Rayborn
    • 1
  • Shreeta Acharya
    • 1
  1. 1.Cole Eye Institute and The Lerner Research InstituteThe Cleveland Clinic FoundationCleveland

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