Development Of Rat Models For Choroidal Neovascularisation (CNV)

A Comparison between Laser and Recombinant Adenovirus Induced CNV
  • P. E. Rakoczy
  • W. Y. Shen
  • K. Spilsbury
  • M. Yu
  • M. Lai
  • C. J. Barry
  • I. J. Constable


This project aimed to investigate whether the overexpression of vascular endothelial growth factor (VEGF) in the retinal pigment epithelium (RPE), induced by recombinant adenovirus mediated delivery of VEGF164, is sufficient to initiate the development of leaky choroidal blood vessels in a rat model. Eyes of pigmented rats were subjected to krypton laser photocoagulation or subretinal injection with recombinant adenovirus constructs Ad.RSV.VEGF or Ad.CMV.VEGF. Initially the animals were examined with fundus photography followed by fluorescein angiography. Retinal photographs demonstrated that the krypton laser produced moderate burns and that the subretinal injection resulted in the formation of a bleb in the subretinal space. Injection of control recombinant adenovirus Ad. RSV.βgal resulted in the expression of βgal reporter gene in the RPE layer. Of the 141 laser spots delivered into a total of 17 rat eyes, 52.4% developed fluorescein leakage that was detectable by angiography. Of seven eyes injected with Ad.RSV.VEGF, 5 developed leakage at 2 weeks post injection, and of 8 eyes which were injected with Ad.CMV.VEGF, 5 developed leakage at 1 week post injection. However, the development of leaky blood vessels was temporary and both Ad.RSV.VEGF and Ad.CMV.VEGF injected animals showed regression of leaky vessels by 4 and 5 weeks post injection, respectively. These results suggest that the upregulation of VEGF in the RPE layer may be a key factor in the series of events leading to the development of CNV.


Vascular Endothelial Growth Factor Retinal Pigment Epithelium Post Injection Recombinant Adenovirus Choroidal Neovascularisation 
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Copyright information

© Kluwer Academic / Plenum Publishers 1999

Authors and Affiliations

  • P. E. Rakoczy
    • 1
  • W. Y. Shen
    • 2
  • K. Spilsbury
    • 2
  • M. Yu
    • 1
  • M. Lai
    • 1
  • C. J. Barry
    • 2
  • I. J. Constable
    • 1
  1. 1.Centre for Ophthalmology and Visual ScienceUniversity of Western AustraliaNedlandsAustralia
  2. 2.Lions Eye InstituteNedlandsAustralia

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