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Host-Vector System and Reverse Genetics in A Non-Conventional Yeast,Candida maltosa

  • Masamichi Takagi

Abstract

In Candida maltosa, expression of many genes is induced by the hydrophobic compound, n-alkane, resulting in biogenesis of organelles such as endoplasmic reticulum and peroxisome where n-alkane is metabolized. Host-vector systems are needed in order to investigate this phenomenon at the molecular level. Since C. maltosa does not have plasmids, the isolation of an ARS site from the genome of C maltosa was attempted so as to construct vectors. Several host-vector systems were constructed to promote reverse genetics in this non-conventional yeast as described in our recent papers shown below.

Keywords

Cloning Vector Reverse Genetic Lithium Acetate Method Candida Maltosa Inducible Cytochrome 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1992

Authors and Affiliations

  • Masamichi Takagi
    • 1
  1. 1.Department of Agricultural ChemistryThe University of TokyoTokyoJapan

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