Recombinant Dna Technology in The Bovine Diseases Control Program and Development of Cattle Industry
For effective control of the vector-borne bovine diseases, a thorough knowledge of the nature of the infective agents, the vectorial capability, and the susceptibility of the bovine host is required. There is, however, little information on prevalence and transmission of bovine diseases due to the difficulty of identifying infected animals by conventional microscopic examination. Therefore, we have used the novel technology of diagnostic DNA-probes for detection of Babesia infection. The best probe can detect as little as 25 ng of purified Babesia DNA, the equivalent of 0.001% parasitemia. Each of the developed probes are species specific. Research on development of vaccines against other tick-borne diseases is also being pursued. The strategy is to identify surface proteins relevant to protection, clone them, and express gene coding for these proteins and test their effectiveness as protective immunogens in recombinant vaccinia construct.
KeywordsParasite Density Bovine Leukemia Virus Infected Blood Merozoite Surface Protein Cattle Industry
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