Conclusions
The increased availability and a variety of recombinant DNA techniques, has provided a route for the genetic analysis of newly isolated bacteria. The data so obtained is essential if the manipulation of metabolic pathways of these strains is to be successful.
Three general conclusions are possible. Using genetically well characterized models, effective procedures for the genetic analysis of newly isolated bacteria can be developed. Next, a combination of physical and genetic techniques provides genetic analysis superior to the more traditional procedures. Finally, improved techniques enables whole genome analysis to be applied to a range of organisms. The unveiling of hidden complexities in the manner in which bacteria store their biological information can only contribute to our ability to manipulate them for practical purposes.
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References
Allen, LN. and Hanson, R.S. (1985). J. Bacteriol. 161, 955–962.
Holloway, B.W., Dharmsthiti, S., Johnson, C, Kearney, A., Krishnapillai, V., Morgan, A.F., Ratnaningsih, E., Saffery, R., Sinclair, M., Strom, D. and Zhang, C. (1990a). In Pseudomonas: Biotransformations, Pathogenesis and Evolving Biotechnology, pp. 269–279. Ed. S. Silver, A. Chakrabarty, B. Iglewski and S. Kaplan. Washington,D.C. American Society for Microbiology.
Holloway, B.W., Bowen, A., Dharmsthiti, S., Krishnapillai, V., Morgan, A., Ratnaningsih, E. and Sinclair, M. (1990b). Genetic tools for the manipulation of metabolic pathways. Proc. 6th Int. Symp. Genet. Indust. Microorganisms, Strasbourg 227–238.
Jeenes, D.J., Soldata, L, Baur, H., Watson, J.M., Mercenier, A., Reimmann, C, Leisinger, T. and Haas, D. (1986). Mol. Gen. Genet. 203, 421–429.
Lyon, B.R., Kearney, P.P., Sinclair, M.I. and Holloway, B.W. (1988). J. Gen. Micro. 134, 123–13
O’Hoy, K. and Krishnapillai, V. (1985). FEMS Micro. Lett. 29, 299–303.
O’Hoy, K. and Krishnapillai, V. (1987). Genetics 115, 611–618.
Ratnaningsih, E., Dharmsthiti, S., Krishnapillai, V., Morgan, A., Sinclair, M. and Holloway, B.W. (1990). J. Gen. Micro. 136, 2351–2357.
Simon, R., Preiefer, U. and Pühler, A. (1983). Bio/Technology 1, 784–791.
Simon, R., O’Connell, M., Labes, M., and Pühler, A. (1986). Meth. Enzymol. 118, 640–659.
Simon, R., Quandt, J. and Klipp, W. (1989). Gene 80, 161–169.
Sinclair, M.I., Maxwell, P.C., Lyon, B.R. and Holloway, B.W. (1986). J. Bacteriol. 168, 1302–1308.
Smith, C.L and Condemine, G. (1990). J. Bacteriol. 172, 1167–1172.
Strom, D.A., Hirst, R., Petering, J. and Morgan, A. (1990). Genetics 126, 497–503.
Suwanto, A. and Kaplan, S. (1989). J. Bacteriol. 171, 5850–5859.
Tsuda, M. and lino, T. (1987). Mol. Gen. Genet. 210, 270–276.
Tsuda, M. and lino, T. (1988). Mol. Gen. Genet. 213, 72–77.
Whitta, S., Sinclair, M.I. and Holloway, B.W. (1985). J. Gen. Micro. 131, 1547–1549.
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© 1992 Plenum Press, New York
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Holloway, W. et al. (1992). Genetic Analysis of Bacterial Strains Freshly Isolated from Natural Sources. In: Mongkolsuk, S., Lovett, P.S., Trempy, J.E. (eds) Biotechnology and Environmental Science. Springer, Boston, MA. https://doi.org/10.1007/978-0-585-32386-2_21
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