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Effects of Electromagnetic Fields On K+(Rb+) Uptake by HeLa Cells

  • Hiroshi Miyamoto
  • Hisao Yamaguchi
  • Toshitaka Ikehara
  • Yosuke Kinouchi

Conclusions

Exposure to strong homogeneous magnetic fields with various magnetic flux densities ofless than 1.6 T had no significant effect on either active or passive Rb+ influxes into HeLa cells at normal or high temperatures. Exposure to a similar magnetic field of 2 T at different temperatures of 10 to 45°C did not cause any change in active or passive Rb+ influx, and no evidence was obtained for the presence of a phase transition point of the cell membrane between 10 and 37°C.

In contrast, exposure to a strong, time-varying magnetic field of quasi-rectangular wave form caused significant inhibition of active Rb+ influx when the frequency of change in the magnetic field was more than 1/20 Hz. Conversely, K+ efflux was stimulated, but passive Rb+ influx was unaffected. Analyses of the amplitudes of the frequency components of the time-varying magnetic field B and its differential dB/dt revealed that B mainly consisted of components with the lowest angular frequencies, whereas dB/dt contained components of various frequencies. The inhibition of active Rb+ influx was not due to change in the cellular ATP content.

Results obtained by micro-fluorometry with fluorescent probes of the membrane potential (diS-C3-(5)) and pH (4-heptadecyl-7-hydroxy-coumarin) showed change in the electrical properties of the cell surface on exposure to the time-varying magnetic field. Results suggested an uneven distribution of electrical charge and an increase in negative charge on the cell surface. The inhibition of active Rb+ influx and the change in electrical properties of the cell membrane were reversible. Further studies are needed to determine whether change in electrical properties is the direct cause of inhibition of the Na+-pump.

Keywords

Magnetic Field HeLa Cell Eddy Current Pulse Magnetic Field Phase Transition Point 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    T. Gualtierotti (1964) Decrease of the sodium pump activity in the frog skin in a steady magnetic field. Physiologist, 7, 150.Google Scholar
  2. 2.
    C. S. Collis, and M. B. Segal (1988) Effects of pulsed electromagnetic fields on Na+ fluxes across stripped rabbit colon epithelium, J. Appl. Physiol., 65, 124–130.Google Scholar
  3. 3.
    M. Hinsenkamp, P. Lheureux, D. Martins (1985) Transmembrane Na/K exchanges under electromagnetic fields. Preliminary study on human erythrocytes. Reconstr. Surg. Traumat., 19. 63–69.Google Scholar
  4. 4.
    R. W. Farndale, A. Maroudas, and T. P. Marsland (1987) Effects of low amplitude pulsed magnetic fields on cellular ion transport. Bioelectromagnetics, 8, 119–143.CrossRefGoogle Scholar
  5. 5.
    A. P. Stevenson, and R. A. Tobey (1985) Potassium ion influx measurements on cultured Chinese hamster cells exposed to 60-Hz electromagnetic fields. Bioelectromagnetics, 6, 189–198.CrossRefGoogle Scholar
  6. 6.
    H. Yamaguchi, T. Ikehara, K. Hosokawa, A. Soda, M. Shono, H. Miyamoto, Y. Kinouchi, and T. Tasaka (1992) Effects of time-varying electromagnetic fields on K+(Rb+) fluxes and surface charge of HeLa cells. Jpn. J. Physiol., 42. 929–943.CrossRefGoogle Scholar
  7. 7.
    H. Miyamoto, L. Rasmussen, and E. Zeuthen (1976) Recording of clonal growth of mammalian cells through many generations, In D. M. Prescott ed. “Methods in Cell Biology”. 13. 107–119. Acad. Press. Inc. New York.Google Scholar
  8. 8.
    H. Miyamoto, T. Sakai, T. Ikehara, and K. Kaniike (1978) Effect of Rb+ substituted for K+ on HeLa cells: cellular content and membrane transport of monovalent cations, and cell growth. Cell Struct. Funct., 3, 313–324.CrossRefGoogle Scholar
  9. 9.
    H. Miyamoto, T. Ikehara, H. Yamaguchi, K. Hosokawa, T. Yonezu, and T. Masuya (1986) Kinetic mechanism of Na+,K+,Cl--cotransport as studied by the Rb+ influx into HeLa cells: effects of extracellular monovalent ions. J. Membrane Biol., 92. 135–150.CrossRefGoogle Scholar
  10. 10.
    T. Ikeharam, T. Sakai, H. Miyamoto, and K. Kaniike (1982) Interrelation between membrane transport and the contents of alkali metal cations in HeLa cells, Jpn. J. Physiol., 32, 13–24.Google Scholar
  11. 11.
    T. Ikehara, H. Yamaguchi, K. Hosokawa, A. Takahashi, and H. Miyamoto (1993) Kinetic study on the effects of intracellular K+ and Na+ on Na+, K+,Cl- cotransport of HeLa cells by Rb+ influx determination, J. Membrane Biol., 132, 115–124.CrossRefGoogle Scholar
  12. 12.
    O. H. Lowry, N. J. Rosebrough, A. L. Farr, and R. J. Randall (1951) Protein measurement with the Folin phenol reagent, J. Biol. Chem., 193, 265–275.Google Scholar
  13. 13.
    S. H. Wright, S. Krasne, I. Kippen, and E. M. Wright (1981) Na+-dependent transport of tricarboxylic acid cycle intermediates by renal brush border membranes. Effects of fluorescence of potential-sensitive cyanine dye, Biochim. Biophys. Acta, 640, 767–778.CrossRefGoogle Scholar
  14. 14.
    R. Pal, W. A. Petri, Jr., Y. Barenolz, and R. R. Wagner (1983) Lipid and protein contributions to the membrane surface potential of vesicular stomatitis virus probed by a fluoresecnt pH indicator, 4-heptadecyl-7-hydroxycoumarin, Biochim. Biophys. Acta, 729, 185–192.CrossRefGoogle Scholar
  15. 15.
    T. Ikehara, H. Yamaguchi, T. Sakai, and H. Miyamoto (1984a) Kinetic parameters and mechanism of active cation transport in HeLa cells as studied by Rb+ influx, Biochim. Biophys. Acta, 775, 297–307.CrossRefGoogle Scholar
  16. 16.
    T. Ikehara, H. Yamaguchi, K. Hosokawa, T. Sakai, and H. Miyamoto (1984) Rb+ influx in response to changes in energy generation: effect of the regulation of the ATP content of HeLa cells, J. Cell. Physiol., 119,273–282.CrossRefGoogle Scholar
  17. 17.
    T. Ikehara, H. Yamaguchi, K. Hosokawa, and H. Miyamoto (1990) Kinetic mechanism of ATP action in Na+-K+-Cl- cotransport of HeLa cells determined by Rb+ influx studies. Am. J. Physiol., 258. C599–C609.Google Scholar
  18. 18.
    T. Ikehara, A. Takahashi, H. Yamaguchi, K. Hosokawa, T. Masuya, and H. Miyamoto (1991) Regulatory changes in the K+, Cl- and water contents of HeLa cells incubated in an isosmotic high K+-medium. Biochim. Biophys. Acta. 1068, 87–96.CrossRefGoogle Scholar
  19. 19.
    A. Takahashi, H. Yamaguchi, and H. Miyamoto (1993) Changes in K+ current of HeLa cells with progression of the cell cycle studied by patch-clamp technique, Am. J. Physiol., 265, C328–C336.Google Scholar
  20. 20.
    Y. Kinouchi, S. Tanimoto, T. Ushita, K. Sato, H. Yamaguchi, and H. Miyamoto (1988) Effects of static magnetic fields on diffusion in solutions. Bioelectromagnetics, 9, 159–166.CrossRefGoogle Scholar
  21. 21.
    T. S. Tenforde, and R. P. Liburdy (1988) Magnetic deformation of phospholipid bilayers: effects on liposome shape and solute permeability at prephase transition temperatures. J. Theoret. Biol., 133. 385–396.CrossRefGoogle Scholar
  22. 22.
    R. P. Liburdy, and P. F. Vanek, Jr. (1985) Microwaves and the cell membrane. II. Temperature, plasma, and oxygen mediate microwave-induced membrane permeability in the erythrocyte. Rad. Res., 102, 190–205.CrossRefGoogle Scholar
  23. 23.
    H. Aceto, Jr., C. A. Tobias, and I. L. Silver (1970) Some studies on the biological effects of magnetic fields. IEEE Trans. Mag. MAG-6, 368–373.CrossRefADSGoogle Scholar
  24. 24.
    M. Blank, and L. Soo (1989) The effects of alternating currents on Na.K-ATPase function. Bioelectrochem. Bioenerg., 22, 313–322.CrossRefGoogle Scholar
  25. 25.
    M. T. Marron, E. M. Goodman, P. T. Sharpe, and B. Greenebaum (1988) Low frequency electric and magnetic fields have different effects on the cell surface. FEBS Lett., 230, 13–16, 1988.CrossRefGoogle Scholar
  26. 26.
    O. M. Smith, E. M. Goodman, B. Greenebaum, and P. Tipnis (1991) An increase in the negative surface charge of U937 cells exposed to a pulsed magnetic field. Bioelectromagnetics. 12, 197–202.CrossRefGoogle Scholar
  27. 27.
    S. McLaughlin, and M.-M. Poo (1981) The role of electro-osmosis in the electric-field-induced movement of charged macromolecules on the surfaces of cells, Biophys. J., 34, 85–93.CrossRefGoogle Scholar

Copyright information

© Plenum Press 1996

Authors and Affiliations

  • Hiroshi Miyamoto
    • 1
  • Hisao Yamaguchi
    • 2
  • Toshitaka Ikehara
    • 2
  • Yosuke Kinouchi
    • 3
  1. 1.Department of Electrical and Electronic Engineering Faculty of EngineeringThe University of TokushimaTokushimaJapan
  2. 2.Department of Life, Environment and Information Faculty of Domestic ScienceTokushima Bunri UniversityTokushimaJapan
  3. 3.Department of Physiology, School of MedicineThe University of TokushimaTokushimaJapan

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