Genetic Analysis of Iron Biomineralization
Establishment of gene transfer has enabled study of analysis of genes in magnetic bacteria and protein production on BMPs using magnetic bacteria as the host cell. Gene transfer into magnetic bacteria, and transposon mutagenesis. are very useful for future analysis of the mechanism of magnetic particle production. The magA gene was the first discoverd gene involved in magnetite biomineralization in magnetic bacteria, and the product of magA seems to be a kind of cation channel protein localized on the cell and magnetic particle membranes. Gene analysis of Magnetospirillum sp. AMB-1 will determine the mechanism.
Moreover, protein expression on the surfaces of magnetic particles using the fusion gene method was studied. Magnetic particles which have fusion proteins on the surfaces can be collected very easily, and production of purified protein will be applied in future.
KeywordsMagnetic Particle Magnetotactic Bacterium magA Gene Promoter Probe Vector MagA Protein
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- 1.Blakemore, R.P., D. Maratea, and R.S. Wolfe, 1979, Isolation and pure culture of a freshwater magnetic spirillum in chemically defined medium, J. Bacteriol. 140:720–729.Google Scholar
- 2.Burgess, J.G., R. Kawaguchi, T. Sakaguchi, R.H. Thornhill, and T. Matsunaga, 1993, Evolutionary relationships among Magnetospirillum strains inferred from phylogenetic analysis of I6S rDNA sequences, J. Bactcriol. 175:6689–6694.Google Scholar
- 4.Djojonegoro, B.M., M.J. Benedik, and R.C. Willson, 1994, Bacteriophage surface display of an immunoglobulin-binding domain of Staphylococcus aureus protein A, Bio Technology 12:169–172.Google Scholar
- 7.Georgiou, G., H.L. Poetschke, C. Stathopoulos, and J.A. Francisco, 1993, Partial application of engineering Gram-negative bacterial cell surfaces, Tibtech 11:6–10.Google Scholar
- 11.Klauser, T., J. Pohlner, and T.F. Meyer, 1990, Extracellular transport of cholera toxin B subunit using Neisseria IgA protease b-domain: conformation-dependent outer membrane translocation, The EMBO Journal 9:1991–1999.Google Scholar
- 13.Matsunaga, T., C. Nakamura, J.G. Burgess, and K. Sode, 1992, Gene transfer in magnetic bacteria; transposon mutagenesis and cloning of genomic DNA fragments required for magnetosome synthesis, J. Bacteriol. 174:2748–2753.Google Scholar
- 20.Schleifer, K.H., D. Schuler, S. Spring, M. Weizenegger, R. Amann, W. Ludwig, and M. Kohler, 1991, The genus Magnetospirillum, gen. nov. Description of Magnetospirillum gryphiswaldense and transfer of Aquaspirillum magnetotacticum to Magnetospirillum magnetotacticum. comb. nov, Syst. Appl. Microbiol. 14:379–385.Google Scholar
- 24.Sode, K., N. Hatano. and M. Tatara. submitted. Cloning of a marine cyanobacterial promoter for foreign gene expression using a promoter probe vector, Appl. Biochem. Biotechnol.Google Scholar
- 25.Tomasiewicz, H.G. and C.S. Mchenry, 1987, Sequence analysis of the Escherichia coli dnaE gene, Journal of Biotechnology 169:5735–5744.Google Scholar
- 26.Waser, M., D. Hess-Bienz, K. Davies, and M. Solioz, 1992, Cloning and disruption of putative NaH-antiporter gene of Enterococus hirae, The Journal of Biological Chemistry 267:5396–5400.Google Scholar