While the majority of cell culture studies use established cell lines, there are some instances in which primary culture is preferred. The desire to study normal terminally differentiated cell types, such as neurons, myocytes, or T cells, in vitro obviously would require primary culture, since these cell types do not divide in vivo. In addition, it has proved difficult with some dividing cells to fully maintain their differentiated function in vitro through multiple passages. Quite often, this is not the result of “dedifferentiation,” as is usually suggested, but rather is due to a deficiency in the culture conditions being used. In any case, there will always be a need to perform primary culture in order to study the properties of cells that are only recently removed from the in vivo situation in order to learn more about their functions in vivo.
KeywordsPrimary Culture Sertoli Cell Tissue Culture Dish Established Cell Line Clara Cell
Unable to display preview. Download preview PDF.
- Barnes, D., Sirbasku, D., and Sato, G. (eds.), 1984a, Methods for Serum-Free Culture of Cells of the Endocrine System, Vol. 2, Alan R. Liss, New York.Google Scholar
- Barnes, D., Sirbasku, D., and Sato, G. (eds.), 1984b, Methods for Serum-Free Culture of Epithelial and Fibroblastic Cells, Vol. 3, Alan R. Liss, New York.Google Scholar
- Barnes, D., Sirbasku, D., and Sato, G. (eds.), 1984c, Methods for Serum-Free Culture of Neuronal and Lymphoid Cells, Vol. 4, Alan R. Liss, New York.Google Scholar
- Freshney, R. I., 1992, Culture of Epithelial Cells, Wiley-Liss, New York.Google Scholar
- Li, R. H., Gao, W.-Q., and Mather, J. P., 1996b, Multiple factors control the proliferation and differentiation of rat early embryonic (day 9) neuroepithelial cells, Endocrine 15:205–217.Google Scholar
- Mather, J. P., and Phillips, D. M., 1984, Primary Culture of Testicular Somatic Cells, in: Barnes, D., Sirbasku, D., and Sato, G. (eds.), Cell culture methods for molecular and cell biology. Vol. II, Alan R. Liss, New York, pp. 29–45.Google Scholar
- Murakami, H., Yamane, I., Barnes, D., Mather, J., Hayashi, I., and Sato, G. (eds.), 1985, Growth and Differentiation of Cells in Defined Environment, Springer-Verlag, New York.Google Scholar
- Piper, H. M. (ed.), 1990, Cell Culture Techniques in Heart and Vessel Research, Springer-Verlag, New York.Google Scholar
- Roberts, P. E., Phillips, D. M., and Mather, J. M., 1990, Properties of a novel epithelial cell from immature rat lung: Establishment and maintenance of the differentiated phenotype, Am. J. Physiol. Lung Cell Mol. Physiol. 3:415–425.Google Scholar
- Roberts, P., Chichester, C., Plopper, C., Lakritz, J., Phillips, D., and Mather, J., 1992, Characterization of an airway epithelial cell from neonatal rat, in: Animal and Cell Technology: Basic and Applied Aspects (H. Murakami, S. Shirahata, and H. Tachibana, eds.), Kluwer Academic Publishers, Boston, pp. 335–341.Google Scholar
- Wood, J. N., 1992, Neuronal Cell Lines: A Practical Approach, Oxford University Press, New York.Google Scholar