The purpose of this chapter is to provide guidelines and suggestions for those investigators wishing to grow cells in hormone-supplemented, serum-free culture. It is neither exhaustive nor definitive. There are two major aims. The first aim is to call to mind specific conditions and viewpoints that may help orient the cell culturist to a slightly different perspective. With this aim in mind, points will be illustrated with results from work done on a specific cell line but which illustrate more general phenomena. The second aim is to cover technical details involved in setting up serum-free experiments, the preparation and handling of hormones, and so on that supplement the standard culture techniques used with traditional serum-containing cultures, which are covered in Chapter 5. Several elements of the role of serum in cell growth media and the substitutes used in serum-free culture are summarized. (See Table 4.3 for commercially available media specifically designed for serum-free culture.)


Fibroblast Growth Factor Schwann Cell Prolactin Secretion Soybean Trypsin Inhibitor Attachment Factor 
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© Plenum Press, New York 1998

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