Advertisement

Abstract

In this section, we will deal with the nutrient mixtures that are usually called media. In actual use, these will almost always be supplemented with serum or another complex biological fluid (milk, embryo extracts, and plasma are examples), or, as discussed in Chapter 8, with a defined mixture of hormones and growth factors. The ongoing experimental work of replacing the complex mixtures with defined components, both nutrients and proteins, largely has been responsible both for our understanding of what the medium does in cell culture and for our increased technical ability to maintain a broad range of functional cells in vitro.

Keywords

Retinoic Acid Nutrient Mixture Pump Head Basal Media Eagle Weak Estrogenic Activity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Biggers, J. D., Gwatrikin, R. B. L., and Heynes, S., 1961, Growth of embryonic avian and mammalian tibiae on a relatively simple chemically defined medium, Exp. Cell Res. 25:41–58.PubMedCrossRefGoogle Scholar
  2. Bottenstein, J., Hayashi, I., Hutchings, S. H., Masui, H., Mather, J., McClure, D. B., Okasa, S., Rizzino, A., Sato, G., Serrero, G., Wolfe, R., and Wu, R., 1979, The growth of cells in serum free hormone supplemented media, Methods Enzymol. 58:94–109.PubMedCrossRefGoogle Scholar
  3. Brewer, G., Torricelli, J., Evege, E., and Price, P. J., 1994, Neurobasal super (TM) medium/B27 supplement: A new serum-free medium combination for survival of neurons, Focus 16:6–8.Google Scholar
  4. Dulbecco, R., and Freeman, G., 1959, Plaque Production by the Polyoma Virus, Virology 8:396–405.PubMedCrossRefGoogle Scholar
  5. Eagle, H., 1955, Nutrition needs of mammalian cells in tissue culture, Science 122:501–504.PubMedCrossRefGoogle Scholar
  6. Eagle, H., 1959, Amino acid metabolism in mammalian cell cultures, Science 130:432–437.PubMedCrossRefGoogle Scholar
  7. Eagle, H., 1965, Propagation in a fluid medium of a human epidermoid carcinoma strain KB, Proc. Exp. Biol. Med. 89:362–364.Google Scholar
  8. Evans, V S., Bryant, J. C., Kerr, H., and Schilling, E., 1964, Chemically defined media for cultivation of long-term cell strains from mammalian species, Exp. Cell Res. 36:439–448.PubMedCrossRefGoogle Scholar
  9. Evans, V. S., Bryant, J. C., Fioramonti, M. C., McQuilkin, W. T., Sanford, K. K., and Earle, W. R., 1956, Studies of nutrient media for tissue cells in vitro. I. A protein free chemically defined medium for cultivation of strain Leydig cells, Cancer Res. 16:77–86.PubMedGoogle Scholar
  10. Fischer, G. A., and Sartorelli, A. C., 1964, Development, Maintenance and Assay of Drug Resistance, in: Eisen, H. N. (ed.), Methods in Medical Research. Vol. 10, Year Book Medical Publishers, Chicago, pp. 247–262.Google Scholar
  11. Ham, R., 1965, Clonal growth of mammalian cells in a chemically defined synthetic medium, Proc. Natl. Acad. Sci. USA 53:288–293.PubMedCrossRefGoogle Scholar
  12. Ham, R. G., 1963, An improved nutrient solution for diploid Chinese hamster and human cell lines, Exp. Cell Res. 29:515–526.PubMedCrossRefGoogle Scholar
  13. Ham, R. G., and McKeehan, W. L. (eds.), 1979, Media and growth requirements, Methods Enzymol. 58:44–93.Google Scholar
  14. Hamilton, W., and Ham, R., 1977, Clonal growth of Chinese hamster cell lines in protein-free media, In Vitro 13:537–547.PubMedCrossRefGoogle Scholar
  15. Knedler, A., and Ham, R. G., 1987, Optimized medium for clonal growth of human microvascular endothelial cells with minimal serum. In Vitro Cell. Dev. Biol. 23:481–491.PubMedCrossRefGoogle Scholar
  16. Leibovitz, A., 1963, The growth and maintenance of tissue-cell cultures in free gas exchange with the atmosphere, Am. J. Hyg. 78:173–180.PubMedGoogle Scholar
  17. Macpherson, I. A., and Stoker, M. 1962, Polyoma transformation of hamster cell clones—An investigation of genetic factors affecting cell competence, Virology 16:147–151.PubMedCrossRefGoogle Scholar
  18. Mather, J., Kaczarowski, E., Gabler, R., and Wilkins, F., 1986, Effects of water purity and additional of common water contaminants on the growth of cells in serum-free media, BioTechniques 4:56–63.Google Scholar
  19. Mather, J. P., Saez, J. M., Dray, F., and Haour, F., 1983, Vitamin E prolongs survival and function of porcine Leydig cells in culture, Ada Endocrinol. 102:470–475.Google Scholar
  20. Mather, J. P., and Sato, G. H., 1979, The growth of mouse melanoma cells in hormone-supplemented, serum-free medium, Exp. Cell Res. 120:191–200.PubMedCrossRefGoogle Scholar
  21. McCoy, T. A., Maxwell, M., and Kruse, P. E., 1959, Amino acid requirements of the Novikoff hepatoma in vitro, Proc. Soc. Exp. Biol. Med. 100:115–118.PubMedGoogle Scholar
  22. McKeehan, W., Hamilton, W., and Ham, R., 1976, Selenium is an essential trace nutrient for growth of WI-38 diploid human fibroblasts, Proc. Natl. Acad. Sci. USA 73:2023–2027.PubMedCrossRefGoogle Scholar
  23. McKeehan, W. L., McKeehan, K. A., Hammond, S. L., and Ham, R. G., 1977. Improved medium for clonal growth of human diploid fibroblasts at low concentrations of serum protein. In Vitro 13:399–416.PubMedCrossRefGoogle Scholar
  24. Moore, G. E., and Kitamura, H., 1968, Cell line derived from patient with myeloma, NY State J. Med. 68:2054–2060.Google Scholar
  25. Morgan, J. R., Morton, H. J., and Parker, R. C., 1950, Nutrition of animal cells in tissue culture. I. Initial studies on a synthetic medium, Proc. Soc. Exp. Biol. Med. 73:1–8.PubMedGoogle Scholar
  26. Parker, R., Castor, L. N., and McCulloch, E. A., 1957, Altered cell strains in continuous culture, NY Acad. Sci. 5:303–313.Google Scholar
  27. Perez-Infante, V., Bardin, C. W., Gunsalus, G. L., Musto, N. A., Rich, K. A., and Mather, J. P., 1986, Differential regulation of testicular transferrin and androgen-binding protein secretion in primary cultures of rat Sertoli cells, Endocrinology 118:383–392.PubMedCrossRefGoogle Scholar
  28. Roberts, P. E., Phillips, D. M., and Mather, J. M., 1990, Properties of a novel epithelial cell from immature rat lung: Establishment and maintenance of the differentiated phenotype, Am. J. Physiol. Lung Cell. Mol. Physiol 3:415–425.Google Scholar
  29. Waymouth, C., 1959, Rapid proliferation of sublines of NCTC Clone 929 (Strain L) mouse cells in a simple chemically defined medium (MB 752/1), J. Natl. Cancer Inst. 22:1003–1015.PubMedGoogle Scholar
  30. Williams, G. M., and Gunn, J. M., 1974, Long-term cell culture of adult rat liver epithelial cells, Exp. Cell Res. 89:139–142.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1998

Personalised recommendations