Cell Culture for Commercial Settings

Part of the Introductory Cell and Molecular Biology Techniques book series (ICMB)


In the last decade, cell culture has come into its own as an area of considerable commercial importance in many biotechnology and pharmaceutical companies (Arathoon and Birch, 1986). The previous chapters have focused on techniques needed in the research laboratory, whether it is located in an academic or commercial setting. These techniques all apply to the cell culture technology that is part of the discovery of new products in biotechnology, whether it is expression cloning of a protein, purification of a desired activity using an in vitro bioassay, the production of a transgenic mouse using embryonic stem (ES) cells, or the optimization of new vectors for mammalian cell expression of recombinant proteins. This chapter will detail some special considerations that apply to cell culture as performed in commercial settings. As with previous chapters on specialized tissue culture techniques, we will not attempt to give complete details on techniques used in this setting, but will refer to published material describing techniques in each subdiscipline.


Mammalian Cell Culture Cell Bank Production Cell Line Roller Bottle Cell Culture Process 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Arathoon, W. R., and Birch, J. R., 1986, Large-scale cell culture in biotechnology, Science 232:1390–1395.PubMedCrossRefGoogle Scholar
  2. Ferrara, N., Leung, D., Cachianes, G., Winer, J., and Henzel, W., 1991, Purification and cloning of vascular endothelial growth factor secreted by pituitary folliculostellate cells, Methods Enzymol 198:391–405.PubMedCrossRefGoogle Scholar
  3. Goeddel, D. V. (ed.), 1991, Methods in Enzymology, Vol. 185, Academic Press, San Diego.Google Scholar
  4. Lubiniecki, A., 1990, Continuous cell substrate considerations, in: Large-Scale Mammalian Cell Culture Technology (A. Lubiniecki, ed.), Marcel Dekker, New York, pp. 495–513.Google Scholar
  5. Mather, J. P., 1990, Optimizing the cell and culture environment for the production of recombinant proteins, Methods Enzymol. 185:157–167.Google Scholar
  6. Mather, J., and Moore, A., 1998. Culture media: Large scale production of proteins in animal cells, in: The Encyclopedia of Bioprocess Technology: Fermentation, Biocatalysis, & Bioseparation (M. Flickinger and S. Drew, eds.), John Wiley & Sons, New York, in press.Google Scholar
  7. Mather, J., and Ullrich, A., 1988, Culturing recombinant host cells by first transforming host cell with nucleic acid encoding polypeptide factor necessary for growth, European Patent # EP 307247.Google Scholar
  8. Pennica, D., Wood, W., and Chien, K., 1996, Cardiotropin-1: A multifunctional cytokine that signals via LIF receptor-gp 130 dependent pathways, Cytokine Growth Factor Rev. 7:81–91.Google Scholar
  9. Portalano, S., McLachlan, S., and Rapoport, B., 1993, High affinity, thyroid specific human autoantibodies displayed on the surface of filamentous phage use V genes similar to other autoantibodies, J. Immunol. 151:2839–2851.Google Scholar
  10. Werther, W., Gonzalez, T., O’Connor, S., McCabe, S., Chan, B., 1996, Humanization of an anti-lymphocyte function-associated antigen (LFA)-l monoclonal antibody and reengineering of the humanized antibody for binding to rhesus LFA-1, J. Immunol. 157:4986–4995.PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1998

Personalised recommendations