Cell Culture for Commercial Settings

Part of the Introductory Cell and Molecular Biology Techniques book series (ICMB)


In the last decade, cell culture has come into its own as an area of considerable commercial importance in many biotechnology and pharmaceutical companies (Arathoon and Birch, 1986). The previous chapters have focused on techniques needed in the research laboratory, whether it is located in an academic or commercial setting. These techniques all apply to the cell culture technology that is part of the discovery of new products in biotechnology, whether it is expression cloning of a protein, purification of a desired activity using an in vitro bioassay, the production of a transgenic mouse using embryonic stem (ES) cells, or the optimization of new vectors for mammalian cell expression of recombinant proteins. This chapter will detail some special considerations that apply to cell culture as performed in commercial settings. As with previous chapters on specialized tissue culture techniques, we will not attempt to give complete details on techniques used in this setting, but will refer to published material describing techniques in each subdiscipline.


Mammalian Cell Culture Cell Bank Production Cell Line Roller Bottle Cell Culture Process 
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© Plenum Press, New York 1998

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