Special Growth Conditions
In this chapter, we will discuss some special considerations for growing cells in different formats from the more typical ones of monolayer plate cultures or small spinner cultures discussed in previous chapters. These will include miniaturizing conditions so that cell culture and growth assays can be adapted to a high-throughput format (Fig. 11.1), which uses a minimum amount of materials while generating quantities of data. The emphasis here is on efficiency, since the limit on how quickly an assay can be run is set by the biological doubling time of the cell. We also discuss scaling up cell culture systems for those applications where a large number of cells or large volumes of conditioned medium are needed for biochemical studies, protein purification, and so forth. These methods are still in the realm of what is desirable for use in a research laboratory. Industrial-scale culture is discussed in Chapter 12. Finally, we discuss growing cells in special configurations that change cell-cell associations (e.g., transwell dishes) or cell shape and cell matrix associations (e.g., in collagen matrix). It should be emphasized again that the increase in complexity of the systems used to grow cells does not alter the basic biology of the cells. All the conditions, caveats, and cautions discussed in earlier chapters still need to be taken into account when working with these more complex culture systems.
KeywordsConditioned Medium Feeder Layer Spinner Base Semisolid Medium Crystal Violet Solution
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