Establishing a Cell Line
Most investigators who use cell culture in their work will obtain their cell lines from other investigators or from a cell bank such as the American Type Culture Collection. It is certainly easier to obtain and use a previously established cell line than to create one. However, there may be instances in which no acceptable line exists with the desired properties, or is derived from the cell type or genetic background of interest. Transgenic and knockout animals also provide the opportunity to establish cell lines with known genetic anomalies. In these cases the investigators may wish to establish their own line in their laboratory. This chapter will deal with the techniques used to establish and characterize new cell lines from normal and transformed tissues.
KeywordsSchwann Cell Pituitary Carcinoma Mouse Embryo Cell Immortal Cell Line Human Schwann Cell
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