Abstract
We have characterized the influence of protein–carbon nanotube interactions on protein structure and function using various techniques such as Fourier transform infrared spectroscopy, circular dichroism spectroscopy, and atomic force microscopy. This structure-based analysis revealed that different proteins interact with nanotubes differentially, consistent with the observed biological activity data. Furthermore, the high degree of surface curvature of the nanoscale support was found to play an important role in stabilizing proteins under denaturing conditions. Along with these fundamental studies, various applications of such highly active and stable nanotube–protein conjugates have been pursued, which include self-cleaning nanobiocomposite films, interfacial biocatalysis in a biphasic medium, and synthesis of nanotube–nanoparticle hybrids, among others.
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- ADH:
-
alcohol dehydrogenase
- AFM:
-
atomic force microscopy
- AGP:
-
α1-acid glycoprotein
- AOT:
-
Aerosol-OT
- BSA:
-
bovine serum albumin
- CALB:
-
Candida antarctica lipase B
- CD:
-
circular dichroism
- CNT:
-
carbon nanotube
- CT:
-
α-chymotrypsin
- d-SBP:
-
deglycosylated soybean peroxidase
- EDC:
-
N-ethyl-N′-(3-dimethylaminopropyl) carbodiimide hydrochloride
- EDX:
-
energy dispersive X-ray
- FAM:
-
carboxyfluorescein
- FT-IR:
-
Fourier transform infrared
- HOPG:
-
highly ordered pyrolytic graphite
- HRP:
-
horseradish peroxidase
- HSA:
-
human serum albumin
- Lys:
-
lysozyme
- MJL:
-
Mucor javanicus lipase
- MWNT:
-
multiwalled nanotube
- NaDDBS:
-
sodium dodecylbenzene sulfonate
- NHS:
-
N-hydroxysuccinimide
- NIR:
-
near infrared
- PAGE:
-
polyacrylamide gel electrophoresis
- PLL:
-
poly-l-lysine
- PMMA:
-
poly(methyl methacrylate)
- PMSF:
-
phenylmethansulfonyl fluoride
- ROS:
-
reactive oxygen species
- SAM:
-
self-assembled monolayer
- SBP:
-
soybean peroxidase
- SC:
-
subtilisin Carlsberg
- SWNT:
-
single-walled nanotube
- TEM:
-
transmission electron microscopy
- TRY:
-
trypsin
- UV:
-
ultraviolet
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Pangule, R.C. et al. (2009). Biomolecule-Nanomaterial Interactions: Effect on Biomolecular Structure, Function, and Stability. In: Puleo, D., Bizios, R. (eds) Biological Interactions on Materials Surfaces. Springer, New York, NY. https://doi.org/10.1007/978-0-387-98161-1_5
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DOI: https://doi.org/10.1007/978-0-387-98161-1_5
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