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Collagen I-Coated Titanium Surfaces for Bone Implantation

  • Marco Morra
  • Clara Cassinelli
  • Giovanna Cascardo
  • Daniele Bollati
Chapter

Abstract

Biological interactions at the tissue/implant material interface can be modulated by surface-linked cell-signalling biological molecules. Collagen type I, the main extracellular matrix protein of bone tissue, has been widely investigated in biomolecular surface modification of bone-contacting titanium implant devices. Literature reports on the biological effects of collagen-based coatings are, however, often contradictory. From a biomolecular surface-engineering perspective, a possible explanation is that the definition “collagen-coated surface” encompasses widely different molecular and supramolecular structures: adsorbed collagen, covalently linked collagen, crosslinked collagen, fibrillar versus monomeric collagen, and many other variation of this theme. Relevant details are not always described and proper surface characterization is often lacking. This chapter attempts to build up a rational frame of reference to describe surface modification of implant devices by collagen type I from a surface chemistry point of view, as well as to discuss relevant implications for process design.

Keywords

Surface Density Collagen Fibril Titanium Surface Collagen Molecule Collagen Coating 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

AFM

atomic force microscopy

Arg–Gly–Asp

arginine –glycine –aspartic acid

BMTiS

biochemical modification of titanium surfaces

Co

cobalt

DAE

double acid etched

ECM

extracellular matrix

EDC

1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide

NHS

N-hydroxysuccinimide

OC

osteocalcin

OP

osteopontin

PBS

phosphate-buffered saline

PDGF

platelet-derived growth factor

PDL

periodontal ligament

PEG

poly(ethylene glycol)

RGD

arginine–glycine–aspartic acid

RT-PCR

real-time polymerase chain reaction

Ti

titanium

Ti6Al4V

titanium/aluminum/vanadium alloy

ToF-SIMS

time-of-flight static secondary ion mass spectroscopy

UHV

ultra-high vacuum

XPS

X-ray photoelectron spectroscopy

Notes

Acknowledgments

Most of this work was performed under the program “Coating bioattivi per dispositivi a contatto con osso” Legge 598/94 art. 11 – Ricerca, Regione Piemonte.

The significant contribution of Dr. Eng. Ilaria Cannella to the analytical work (which was part of her thesis work) is acknowledged.

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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Marco Morra
    • 1
  • Clara Cassinelli
    • 1
  • Giovanna Cascardo
    • 1
  • Daniele Bollati
    • 1
  1. 1.Nobil Bio Ricerche s.r.lPortacomaroItaly

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