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Toward Osteogenic Differentiation of Marrow Stromal Cells and In Vitro Production of Mineralized Extracellular Matrix onto Natural Scaffolds

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Biological Interactions on Materials Surfaces

Abstract

Tissue engineering has emerged as a new interdisciplinary field for the repair of various tissues, restoring their functions by using scaffolds, cells, and/or bioactive factors. A temporary scaffold acts as an extracellular matrix analog to culture cells and guide the development of new tissue. In this chapter, we discuss the preparation of naturally derived scaffolds of polysaccharide origin, the osteogenic differentiation of mesenchymal stem cells cultured on biomimetic calcium phosphate coatings, and the delivery of biomolecules associated with extracellular matrix mineralization.

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Abbreviations

BMP:

bone morphogenetic protein

BMP-2:

bone morphogenetic protein-2

BMSC:

bone marrow stromal cell

BMSSC:

bone marrow stromal stem cell

CaP:

calcium phosphate

ECM:

extracellular matrix

FGF-1:

fibroblast growth factor-1

FGF-2:

fibroblast growth factor-2

IGF-2:

insulin-like growth factor-2

MSC:

mesenchymal stem cell

RGD:

arginine–glycine–aspartic acid

SBF:

simulated body fluid

SPCL:

blend of starch and poly(ε-caprolactone)

TGF-β:

transforming growth factor-β

TGF-β1:

transforming growth factor-β1

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Acknowledgments

The authors would like to acknowledge European NoE EXPERTISSUES (NMP3-CT-2004-500283) (R.L.R), Project HIPPOCRATES (NMP3-CT-2003-505758) (R.L.R), and grants from the US National Institutes of Health to A.G.M. (R01 AR42639, R01 DE15164 and R01 DE17441).

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  1. 3B’s Research Group – Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, 4806-909, Taipas, Guimarães, Portugal

    Ana M. Martins, Catarina M. Alves & Rui L. Reis

  2. IBB – Institute for Biotechnology and Bioengineering, PT Government Associated Laboratory, Guimarães, Portugal

    Ana M. Martins, Catarina M. Alves & Rui L. Reis

  3. Department of Bioengineering, Rice University, 6100 Main Street, Houston, TX, 77005-1892, USA

    Ana M. Martins, Antonios G. Mikos & F. Kurtis Kasper

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  1. Ana M. Martins
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    Martins, A.M., Alves, C.M., Reis, R.L., Mikos, A.G., Kasper, F.K. (2009). Toward Osteogenic Differentiation of Marrow Stromal Cells and In Vitro Production of Mineralized Extracellular Matrix onto Natural Scaffolds. In: Puleo, D., Bizios, R. (eds) Biological Interactions on Materials Surfaces. Springer, New York, NY. https://doi.org/10.1007/978-0-387-98161-1_13

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