Toward Osteogenic Differentiation of Marrow Stromal Cells and In Vitro Production of Mineralized Extracellular Matrix onto Natural Scaffolds

  • Ana M. Martins
  • Catarina M. Alves
  • Rui L. Reis
  • Antonios G. Mikos
  • F. Kurtis Kasper


Tissue engineering has emerged as a new interdisciplinary field for the repair of various tissues, restoring their functions by using scaffolds, cells, and/or bioactive factors. A temporary scaffold acts as an extracellular matrix analog to culture cells and guide the development of new tissue. In this chapter, we discuss the preparation of naturally derived scaffolds of polysaccharide origin, the osteogenic differentiation of mesenchymal stem cells cultured on biomimetic calcium phosphate coatings, and the delivery of biomolecules associated with extracellular matrix mineralization.


Tissue Engineering Osteogenic Differentiation Simulated Body Fluid Bioactive Glass Bone Tissue Engineering 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



bone morphogenetic protein


bone morphogenetic protein-2


bone marrow stromal cell


bone marrow stromal stem cell


calcium phosphate


extracellular matrix


fibroblast growth factor-1


fibroblast growth factor-2


insulin-like growth factor-2


mesenchymal stem cell


arginine–glycine–aspartic acid


simulated body fluid


blend of starch and poly(ε-caprolactone)


transforming growth factor-β


transforming growth factor-β1



The authors would like to acknowledge European NoE EXPERTISSUES (NMP3-CT-2004-500283) (R.L.R), Project HIPPOCRATES (NMP3-CT-2003-505758) (R.L.R), and grants from the US National Institutes of Health to A.G.M. (R01 AR42639, R01 DE15164 and R01 DE17441).


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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Ana M. Martins
    • 1
    • 2
    • 3
  • Catarina M. Alves
    • 1
    • 2
  • Rui L. Reis
    • 1
    • 2
  • Antonios G. Mikos
    • 3
  • F. Kurtis Kasper
    • 3
  1. 1.3B’s Research Group – Biomaterials, Biodegradables and BiomimeticsUniversity of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative MedicineTaipasPortugal
  2. 2.IBB – Institute for Biotechnology and BioengineeringPT Government Associated LaboratoryGuimarãesPortugal
  3. 3.Department of BioengineeringRice UniversityHoustonUSA

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