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Development and Differentiation of Neural Stem and Progenitor Cells on Synthetic and Biologically Based Surfaces

  • Erin N. Boote Jones
  • Donald S. Sakaguchi
  • Surya K. Mallapragada
Chapter

Abstract

The response of stem and progenitor cells to material surfaces is of particular interest to the fields of biomedical engineering and regenerative medicine for a number of reasons. It is thought that stem and progenitor cells may represent a new treatment option for diseases of large-scale cell loss or malfunction, particularly in the central nervous system, where the environment is not permissive of tissue regeneration. Before treatments with specific outcomes can be reliably planned, however, the interactions between stem and progenitor cells and their environment must be better understood. This chapter discusses the function of neural stem and progenitor cells, as well as their responses to nonbiodegradable and biodegradable polymers, and to biologically derived proteins, which are used both as coatings on material surfaces and as scaffolds.

Keywords

Nerve Growth Factor Glial Fibrillary Acidic Protein Neurite Outgrowth Alginate Hydrogel Alginate Lyase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

AEMA

aminoethyl methacrylate

AHPC

adult hippocampal progenitor cell

AL

alginate lyase

AMF

aligned microfiber

ANF

aligned nanofiber

C17.2

immortalized neural stem cell line from murine postnatal cerebellum

CE3

murine embryonic stem cell line expressing green fluorescent protein

CNPase

oligodendrocyte marker

CNS

central nervous system

Dex-MA

methacrylated dextran

EB

embryoid body

ECM

extracellular matrix

EVAL

poly(ethylene-co-vinyl alcohol)

FMCC

fetal mouse cortical cells

GFAP

glial fibrillary acidic protein

GP

glycerophosphate salt

HiB5

rat hippocampal progenitor cell line

IKVAV

peptide sequence isoleucine–lysine–valine–alanine–valine

K14

keratin-14, a long α-helical peptide

LAS

lysine-alanine sequential polymer

LG3

a globular domain of the laminin α3 chain

LG3K14

a polypeptide composed of keratin-14 fused to the LG3 domain

MC

methacrylamide chitosan

MTT

colorimetric mitochondrial metabolic activity assay

NeuN

mature neuron marker

NGF

nerve growth factor

NPC

neural progenitor cell

NSPC

neural stem and progenitor cell

NT-3

neurotrophin-3

PAA-azido

N-4-(azidobenzoyloxy)succinimide conjugated to polyallylamine

PC12

rat cancer cell line derived from a pheochromocytoma of the adrenal medulla

PDL

poly(d-lysine)

PEG

poly(ethylene glycol)

PLA

poly(lactic acid)

PLCL

poly(l-lactide-co-ε-caprolactone)

PLGA

poly(lactic-co-glycolic acid)

PLL

poly(l-lysine)

PLLA

poly(l-lactic acid)

PPy

polypyrrole

PVA

poly(vinyl alcohol)

RA

retinoic acid

RGD

peptide sequence arginine-glycine-aspartate

RMF

random microfiber

RNF

random nanofiber

RW4

murine embryonic stem cell line

SEM

scanning electron microscope

TERA2.cl.SP12

human pluripotent embryonic carcinoma cells

TUJ1

class III β-tubulin

TUNEL

terminal deoxynucleotidyl transferase dUTP nick end labeling

YIGSR

peptide sequence tyrosine–isoleucine–glycine–serine–arginine

Notes

Acknowledgments

The authors gratefully acknowledge the NIH (RO1GM072005) for financial support.

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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Erin N. Boote Jones
    • 1
  • Donald S. Sakaguchi
    • 2
  • Surya K. Mallapragada
    • 1
  1. 1.Department of Chemical and Biological EngineeringIowa State UniversityAmesUSA
  2. 2.Department of Genetics, Development, and Cell BiologyIowa State UniversityAmesUSA

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