Organotypic Models of Metastasis: A Three-dimensional Culture Mimicking the Human Peritoneum and Omentum for the Study of the Early Steps of Ovarian Cancer Metastasis
Because most ovarian cancer (OvCa) patients present at a late stage, when metastasis has already occurred, the study of early events in peritoneal dissemination is difficult. One problem has been the lack of adequate model systems for the study of ovarian tumor transformation and metastasis.1,2 Current models in use include co-cultures, whole tissue cultures, and immunocompromised and genetic mouse models. All of these have unique advantages; however, none of them replicates the human in vivo situation. The development and use of a three-dimensional (3D) organotypic model of OvCa has the potential to bridge the gap between the current models of OvCa and the human disease.
Ovarian Cancer Metastasis
Most patients with OvCa present with advanced disease metastatic to the peritoneum. Despite aggressive surgery and chemotherapy, patients with intra-abdominal, widely disseminated OvCa rarely achieve long-term cures.3The key to improved treatment of OvCa is a better...
KeywordsMesothelial Cell OvCa Cell OvCa Cell Line Human Mesothelial Cell Omental Metastasis
The development of the 3D ovarian cancer culture was supported over the years through grants to Ernst Lengyel from the Gynecologic Cancer Foundation (2005/2006 GCF/Molly Cade Ovarian Cancer Research Grant), the Ovarian Cancer Research Fund (OCRF, Liz Tilberis Scholars Program), and the NCI (R01 CA111882). Ernst Lengyel holds a Clinical Scientist Award in Translational Research from the Burroughs Wellcome Fund. Hilary A. Kenny was supported by a Penny Severns Breast, Cervical, and Ovarian Cancer Research postdoctoral fellowship from the Illinois Department of Public Health and a Graduate Training Program in Cancer Biology postdoctoral fellowship through the University of Chicago (NIH/NCI 5T32 CA09594). Songuel Dogan was supported by the Deutsche Forschungsgemeinschaft (German Research Council) DOI309/1–1. Marion Zillhardt was supported by the Graduate Training Program in Cancer Biology through the University of Chicago (NIH/NCI T32 CA09594). The authors would like to thank Gail Isenberg (University of Chicago) for her graphic designs and editorial expertise.
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