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Advanced Photonic Structures for Biological and Chemical Detection pp 487–512Cite as

Single Molecule Analysis with Planar Optofluidics

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Part of the book series: Integrated Analytical Systems ((ANASYS))

Abstract

Integrated optofluidics uses integrated optical elements for building a new generation of analytical devices that feature planar architectures for both fluidics and optics. Liquid-core optical waveguides that can simultaneously guide both liquids and light through micron-scale hollow channels are essential components of such a platform. Here, we review advances in single particle detection, manipulation, and analysis in integrated optofluidic chips based on liquid-core antiresonant reflecting optical waveguides (ARROWs). Starting from a discussion of physical principles and fabrication methods, we discuss demonstrations of liquid-core waveguiding, fluorescence and Raman detection, single molecule analysis, and all-optical particle manipulation. Special emphasis is placed on the on-chip implementation of fluorescence correlation spectroscopy for analysis of biological particles, including liposomes and viruses. An outlook on future opportunities and challenges is given.

The online version of the Erratum chapter can be found at http://dx.doi.org/10.1007/978-0-387-98063-8_20

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Acknowledgments

Many students and colleagues have contributed to the research described in the preceding sections. It is a great pleasure to acknowledge the contributions of D. Yin, J.P. Barber, D.W. Deamer, P. Measor, E.J. Lunt, S. Kühn, M.I. Rudenko, M. Smith, B.S. Phillips, M.R. Holmes, D. Ermolenko, H.F. Noller, L. Seballos, J. Zhang, M.G. Finn, U. Håkanson, and V. Sandoghdar. The different aspects of this work were enabled by financial support from the National Institutes of Health (grants R21EB003430 and R01EB006097), the National Science Foundation (grants ECS-0528730 and ECS-0528714), the W.M. Keck Foundation (National Academies Keck Futures Initiative Award NAKFI-Nano14), the California Systemwide Biotechnology Research & Education Program Training Program (grant UC-GREAT 2005–245), NASA (NASA/UARC Aligned Research Program (ARP) grant), the D. Huber Foundation, and Ted Goldstein.

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  1. School of Engineering, University of California Santa Cruz, 1156 High St., Santa Cruz, CA, 95064, USA

    Holger Schmidt

  2. ECEn Department, Brigham Young University, 459 Clyde Building, Provo, UT, 84602, USA

    Aaron R. Hawkins

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  1. Holger Schmidt
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  2. Aaron R. Hawkins
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  1. Dept. Biological Engineering, University of Missouri, 240D Bond Life Sciences Center, 1201 E. Rollins St., 65211, Columbia, MO, USA

    Xudong Fan

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Schmidt, H., Hawkins, A.R. (2009). Single Molecule Analysis with Planar Optofluidics. In: Fan, X. (eds) Advanced Photonic Structures for Biological and Chemical Detection. Integrated Analytical Systems. Springer, New York, NY. https://doi.org/10.1007/978-0-387-98063-8_18

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