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Clathrin-Mediated Endocytosis

  • Chapter
Trafficking Inside Cells

Part of the book series: Molecular Biology Intelligence Unit ((MBIU))

Abstract

Eukaryotic cells use multiple pathways for the endocytic entry of proteins and lipids at the plasma membrane. To date, the best characterized pathway is clathrin-mediated endocytosis. This chapter presents an overview of the mechanisms of clathrin-mediated endocytosis and how itis regulated. We provide a mechanistic description of how a clathrin-coated vesicle (CCV) is formed, from the stages of initiation to scission to uncoating, as well as address important regulation by protein and lipid kinases and phosphatases. Endocytic events are initiated through the concerted action of the clathrin coat and adaptor proteins that select the transmembrane proteins (cargo) that will be carried into the cell in endocytic vesicles. Accessory proteins and the GTPase dynamin work together with forces provided by actin polymerization to complete the formation of the CCV. The ATPase chaperone Hsc70 and the protein auxilin promote CCV uncoating, a necessary step for the vesicle to fuse with endosomes. The synergistic convergence of powerful experimental strategies such as structural, biochemical and genomic approaches, in vitro assays, and real-time imaging in vivo, have combined to allow the new breakthroughs that are discussed.

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Authors and Affiliations

  1. Department of Biology, The Johns Hopkins University, 3400 North Charles Street, Baltimore, MD, 21218, USA

    Beverly Wendland

  2. Department of Neurology and Neurosurgery Montreal Neurological Institute, McGill University, Montreal, Quebec, Canada

    Peter S. McPherson & Brigitte Ritter

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  1. Peter S. McPherson
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McPherson, P.S., Ritter, B., Wendland, B. (2009). Clathrin-Mediated Endocytosis. In: Trafficking Inside Cells. Molecular Biology Intelligence Unit. Springer, New York, NY. https://doi.org/10.1007/978-0-387-93877-6_9

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