Proteinase Optical Imaging Tools for Cancer Detection and Response to Therapy



A variety of physiological processes such as wound healing and tissue remodeling are mediated by a plethora of proteinases – enzymes that can hydrolyze peptide bonds – of which as many as 622 have been identified in the human genome. These proteinases are classified into five of the seven clans of peptidases with known catalytic type: S (serine), C (cysteine), A (aspartyl), M (metallo), and T (threonine) [MEROPS,; (Rawlings et al. 2008) (Fig. 1)]. In many physiological processes, the proteinases mediate and/or regulate both intercellular signaling, such as in the release and/or processing of chemokines, and intracellular pathways, such as in the apoptotic pathways leading to programmed cell death. Dysregulation of the temporal and/or spatial co-ordination of these intracellular and/or intercellular pathways disrupts the normal physiology and rhythm of life that can be manifest in unregulated growth such as occurs in tumors and their metastatic progeny.


Reactive Functional Group Serine Hydrolase Proteolytic Cascade Hydrolyze Peptide Bond Optical Contrast Agent 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



We thank Randy Scherer for the images of tumors and adenomas with proteolytic beacons. This work was supported by National Institutes of Health grant CA60867 to L.M.M., grant P30 068485 to the Vanderbilt-Ingram Cancer Center, and grant U24 CA126588, the Southeastern Center for Imaging Animal Models, Vanderbilt University Institute of Imaging Science.


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Authors and Affiliations

  1. 1.Department of Cancer Biology, and Vanderbilt–Ingram Cancer Center and Vanderbilt University Institute of Imaging ScienceVanderbilt University, Medical CenterNashvilleUSA
  2. 2.Department of Cancer Biology, and Vanderbilt-Ingram Cancer CenterVanderbilt University Medical CenterNashvilleUSA

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