Fluorophores for Optical Imaging
Optical imaging using fluorescent probes, along with related biomedical imaging technologies such as PET, SPECT, MRI and bioluminescence, represents a return to in situ observational methods of biological discovery in contrast to the reductionist molecular level genomic and proteomic methods that have dominated biomedical research for the past 50 years. This chapter will delineate and compare the properties of fluorescent probes, which are the molecular constructs responsible for contrast generation in most fluorescence imaging applications. For this reason, fluorescence microscopy and other imaging technologies that can deliver this essential contextual information are of ever-increasing importance in biomedical research. This chapter will delineate and compare the properties of fluorescent probes, which are the molecular constructs responsible for contrast generation in most fluorescence imaging applications.
KeywordsFluorescent Probe Fluorescence Quantum Yield Multiplex Detection Contrast Generation Surface Coating Layer
- Alam A, Chavan AJ, Flower RW. (2005) US Patent 6,944,493.Google Scholar
- Megason S, Amsterdam A, Hopkins N, Lin S. (2006) Green Fluorescent Protein: Properties, Applications and Protocols, 2nd Edn (Chalfie M, Kain SR, Eds.) Wiley-Liss, Wilmington, DE, pp. 285–303.Google Scholar
- Ryman-Rasmussen JP, Riviere JE, Monteiro-Riviere NA. (2007) J Invest Dermatol. 127:143–153.Google Scholar
- Seleverstov O, Zabirnyk O, Zscharnack M, Bulavina L, Nowicki M, Heinrich JM, Yezhelyev M, Emmrich F, O’Regan R, Bader A. (2006) Nano Lett. 6:2826–2832.Google Scholar
- Snapp E. (2005) Current Protocols in Cell Biology, John Wiley & Sons, Inc., New York, pp. 21.4.1–21.4.13.Google Scholar