Organic Compounds used in Aquaculture

Part of the Emerging Topics in Ecotoxicology book series (ETEP, volume 1)


The transport patterns and potential direct or indirect effects on the environment or even on human health of agents used in aquaculture like antibiotics, triphenylmethane dyes and hormones used to modify sex ratios have barely been defined. Even at trace levels, hormones may influence the endocrine systems of non-target organisms due to their high biological low-dose activity. Antibiotics are often also active at low concentrations but by their nature do not directly affect benthic species. Nevertheless, prophylactic use of antimicrobials in aquacultures may result both in environmental or human health risks. Potential risks are primarily the direct or indirect transfer of resistant bacteria generated by antibiotic treatment and the development of reservoirs of transferable resistance genes in bacteria in aquatic environments. Another important issue is the occurrence of veterinary drugs in fish and fish products intended for human consumption. Illegal uses, and also environmental background contaminations from legal sources, may lead to residues that might be unacceptable with regard to consumer health for toxicological reasons or simply for legal reasons such as violation of zero tolerances.


Malachite Green Crystal Violet European Food Safety Authority Veterinary Drug Shrimp Pond 
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  1. Alderman DJ, Hastings TS (1998) Antibiotic use in aquaculture. development of antibiotic resistance – potential for consumer health risks. Int J Food Sci Technol 33:139–155CrossRefGoogle Scholar
  2. Allen YT, Thain JE, Haworth S, Barry J (2007) Development and application of long-term sublethal whole sediment tests with Arenicola marina and Corophium volutator using Ivermectin as the test compound, Environ Pollut 146:92–99CrossRefGoogle Scholar
  3. Ankley GT, Jensen KM, Kahl MD, Korte JJ, Makynen EA (2001) Description and evaluation of a short-term reproduction test with the fathead minnow (Pimephales promelas). Environ Toxicol Chem 20:1276–1290Google Scholar
  4. Banasiak U, Heseker H, Sieke C, Sommerfeld C, Vohmann C (2005) Abschätzung der Aufnahme von Pflanzenschutzmittel-Rückständen in der Nahrung mit neuen Verzehrsmengen für Kinder. Bundesgesundheitsbl Gesundheitsforsch Gesundheitsschutz 48:84–98CrossRefGoogle Scholar
  5. Bauer K, Dangschat H, Knöppler H-O (1988) Aufnahme und Ausscheidung von Malachitgrün bei Regenbogenforellen. Arch Lebensmittelhyg 39:97–102Google Scholar
  6. Cabello FC (2006) Heavy use of prophylactic antibiotics in aquaculture: a growing problem for human and animal health and for the environment. Environ Microbiol 8:1137–1144CrossRefGoogle Scholar
  7. Capone DG, Weston DP, Miller V, Shoemaker C (1996) Antibacterial residues in marine sediments and invertebrates following chemotherapy in aquaculture. Aquaculture 145:55–75CrossRefGoogle Scholar
  8. Collier LM, Pinn EH (1998) An assessment of the acute impact of the sea lice treatment ivermectin on a benthic community. J Exp Mar Biol Ecol 230:131–147CrossRefGoogle Scholar
  9. Culp SJ, Mellick PW, Trotter RW, Greenlees K–J, Kodell RL, Beland FA (2006) Carcinogenicity of malachite green chloride and leucomalachite green in B6C3F1 mice and F344 rats. Food Chem Toxicol 44:1204–1212CrossRefGoogle Scholar
  10. Dabrowski K, Rodriguez G, Lee K-J, Abiado MAG, Sanchez WC, Marquez-Couturier G, Phelps R (2004) Studies on fate of methyltestosterone and its metabolites in tilapia and on the use of phytochemicals as an alternative method to produce a monosex population of tilapia. In: Harris R, Courter I, Egna H (eds) Twenty-first annual technical report. Aquaculture CRSP, Oregon State University, Corvallis, OR, pp 53–60Google Scholar
  11. Davies IM, Gillibrand PA, McHenery JG, Rae GH (1998) Environmental risk of ivermectin to sediment dwelling organisms. Aquaculture 163:29–46CrossRefGoogle Scholar
  12. EFSA (European Food Safety Authority) (2005a) Opinion of the Scientific Panel on food additives, flavourings, processing aids and materials in contact with food (AFC) to review the toxicology of a number of dyes illegally present in food in the EU. EFSA J 263:1–71Google Scholar
  13. EFSA (European Food Safety Authority) (2005b) Opinion of the Scientific Committee on a request from EFSA related to a harmonised approach for risk assessment of substances which are both genotoxic and carcinogenic. EFSA J 282:1–31Google Scholar
  14. EU (European Union) (1998) Conference on the microbial threat. The Copenhagen recommendations. Report from the invitational EU conference on the microbial threat, Copenhagen, Denmark, 9–10 Sept 1998 as cited in FAO (2005)Google Scholar
  15. European Communities (2007) The Rapid Alert System for Food and Feed (RASFF): Annual Report 2006, Office for Official Publications of the European Communities, Luxembourg, Accessed 04 Sept 2008
  16. European Communities (2008) The Rapid Alert System for Food and Feed (RASFF): Annual Report 2007, Office for Official Publications of the European Communities, Luxembourg Accessed 04 Sept 2008
  17. FAO (2005) Responsible use of antibiotics in aquaculture. FAO fisheries technical paper no. 469. Food and Agriculture Organization of the United Nations (FAO), RomeGoogle Scholar
  18. FAO (2006) The state of world fisheries and aquaculture 2006. FAO fisheries and aquaculture department, FAO fisheries technical paper no. 500. Food and Agriculture Organization of the United Nations (FAO), RomeGoogle Scholar
  19. Francis-Floyd R, Reed P (2002) Ichthyophthirius multifiliis (white spot) infections in fish. CIR920 of the Fisheries and Aquatic Sciences Department Series, Florida Cooperative Extension Service, Institute of Food and Agricultural Sciences, University of Florida 1991, revised July, 2002. Accessed 06 May 2008
  20. Giraud E, Douet DG, Le Bris H, Bouju-Albert A, Donnay-Moreno C, Thorin C, Pouliquen H (2006) Survey of antibiotic resistance in an integrated marine aquaculture system under oxolinic acid treatment. FEMS Microbiol Ecol 55:439–448CrossRefGoogle Scholar
  21. Green BW, Teichert Coddington DR (2000) Human food safety and environmental assessment of the use of 17 alpha-methyltestosterone to produce male tilapia in the United States. J World Aquacult Soc 31:337–357CrossRefGoogle Scholar
  22. Halling-Sørensen B, Nors Nielsen S, Lanzky PF, Ingerslev F, Holten Lützhøft HC, Jørgensen SE (1998) Occurrence, fate and effects of pharmaceutical substances in the environment – a review. Chemosphere 36:357–393CrossRefGoogle Scholar
  23. Hamscher G (2006) Veterinary pharmaceuticals. In: Reemtsma T, Jekel M (eds) Organic pollutants in the water cycle. Wiley-VCH, Weinheim, pp 99–120CrossRefGoogle Scholar
  24. Heberer T, Batt N (2007) Risk assessment of malachite green residues - literature study (prepared by Germany). Agenda Item 9, CRD 9, Joint FAO/WHO Food Standards Programme Codex Committee on Residues of Veterinary Drugs in Foods. 17th Session, Breckenridge, Colorado, USAGoogle Scholar
  25. Heberer T, Lahrssen-Wiederholt M, Schafft H, Abraham K, Pzyrembel H, Henning KJ, Schauzu M, Braeuning J, Goetz M, Niemann L, Gundert-Remy U, Luch A, Appel B, Banasiak U, Böl GF, Lampen A, Wittkowski R, Hensel A (2007) Zero tolerances in food and animal feed – Are there any scientific alternatives? A European point of view on an international controversy. Toxicol Lett 175:118–135CrossRefGoogle Scholar
  26. Hernando MD, De Vettori S, Martínez Bueno MJ, Fernández-Alba AR (2007) Toxicity evaluation with Vibrio fischeri test of organic chemicals used in aquaculture. Chemosphere 68:724–730CrossRefGoogle Scholar
  27. Ho S-P, Hsu T-Y, Che M-H, Wang W-S (2000) Antibacterial effect of chloramphenicol, thiamphenicol and florfenicol against aquatic animal bacteria. J Vet Med Sci 62:479–485CrossRefGoogle Scholar
  28. Holmström K, Gräslund S, Wahlström A, Poungshompoo S, Bengtsson B-E, Kautsky N (2003) Antibiotic use in shrimp farming and implications for environmental impacts and human health. Int J Food Sci Technol 38:255–266CrossRefGoogle Scholar
  29. Jacobsen P, Berglind L (1988) Persistence of oxytetracycline in sediments from fish farms. Aquaculture 70:365–370CrossRefGoogle Scholar
  30. Kennedy G, Cannavan A, McCracken RJ (2000) Regulatory problems caused by contamination, a frequently overlooked cause of veterinary drug residues. J Chromatogr A 882:37–52CrossRefGoogle Scholar
  31. Kerry J, Hiney M, Coyne R, NicGabhainn S, Gilroy D, Cazabon D, Smith P (1995) Fish feed as a source of oxytetracycline-resistant bacteria in the sediments under fish farms. Aquaculture 131:101–113CrossRefGoogle Scholar
  32. Lalumera GM, Calamari D, Galli P, Castiglioni S, Crosa G, Fanelli R (2004) Preliminary investigation on the environmental occurrence and effects of antibiotics used in aquaculture in Italy. Chemosphere 54:661–668CrossRefGoogle Scholar
  33. LAVES (2005a) Malachitgrün in Forellenkaviar nachgewiesen. Press release of the Niedersächsisches Landesamt für Verbraucherschutz und Lebensmittelsicherheit (LAVES). Accessed 04 Sept 2008
  34. LAVES (2005b) Weihnachts“karpfen: aus niedersächsischen Aquakulturen auf den Festtags-Tisch (in German). Press release No. 74 of the Niedersächsisches Landesamt für Verbraucherschutz und Lebensmittelsicherheit (LAVES). Accessed 04 Sept 2008
  35. LAVES (2008) LAVES untersucht Fisch aus Aquakulturen auf Rückstände. Press release of the Niedersächsisches Landesamt für Verbraucherschutz und Lebensmittelsicherheit (LAVES). Accessed 04 Sept 2008
  36. Le TX, Munekage Y (2004) Residues of selected antibiotics in water and mud from shrimp ponds in mangrove areas in Viet Nam. Mar Pollut Bull 49:922–929CrossRefGoogle Scholar
  37. Le TX, Munekage Y, Kato S (2005) Antibiotic resistance in bacteria from shrimp farming in mangrove areas. Sci Total Environ 349:95–105CrossRefGoogle Scholar
  38. Midtlyng PJ (2000) Vaccination in salmonid aquaculture: A review. In: Fingerman M, Nagabhushnam R (eds) Recent advances in marine biotechnology. vol. 5. Immunobiology and pathology. Science Publishers, Enfield, NH, pp 227–242Google Scholar
  39. Miranda CD, Zemelman R (2001) Antibiotic resistant bacteria in fish from the Concepcion Bay, Chile. Mar Pollut Bull 42:1096–1102CrossRefGoogle Scholar
  40. Nawaz MS, Erickson BD, Khan AA, Khan SA, Pothulari JV, Rafii F, Sutherland JB, Wagner RD, Cerniglia CE (2001) Human health impact and regulatory issues involving antimicrobial resistance in the food animal production environment. Reg Res Perspect 1:1–10Google Scholar
  41. Olah J, Farkas J (1978) Effect of temperature, pH, antibiotics, formalin and malachite green on the growth and survival of Saprolegnia and Achlya parasitic on fish. Aquaculture13: 273–288.CrossRefGoogle Scholar
  42. Orn S, Holbech H, Madsen TH, Norrgren L, Petersen GI (2003) Gonad development and vitellogenin production in zebrafish (Danio rerio) exposed to ethinylestradiol and methyltestosterone. Aquatic Toxicol 65:397–411CrossRefGoogle Scholar
  43. PAN (2003) Chemikalieneinsatz in der Aquakultur. Pestizid Aktions-Netzwerk e.V. (PAN Germany), GTZ (GmbH), pp 90Google Scholar
  44. Penders J, Stobberingh EE (2008) Antibiotic resistance of motile aeromonads in indoor catfish and eel farms in the southern part of The Netherlands. Int J Antimicrobia Agents 31:261–265CrossRefGoogle Scholar
  45. Pérez-Estrada LA, Agüera A, Hernando MD, Malato S, Fernández-Alba AR (2008) Photodegradation of malachite green under natural sunlight irradiation: Kinetic and toxicity of the transformation products. Chemosphere 70:2068–2075CrossRefGoogle Scholar
  46. Plakas SM, El Said KR, Stehly R, Gingerich WH, Allen JL (1996) Uptake, tissue distribution, and metabolism of malachite green in the channel catfish (Ictalurus punctatus). Can J Fish Aquat Sci 53:1427–1433CrossRefGoogle Scholar
  47. Rigos G, Nengas I, Alexis M, Troisi GM (2004) Potential drug (oxytetracycline and oxolinic acid) pollution from Mediterranean sparid fish farms. Aquatic Toxicol 69:281–288CrossRefGoogle Scholar
  48. Richardson ML, Bowron JM (1985) The fate of pharmaceutical chemicals in the aquatic environment. J Pharm Pharmacol 37:1–12CrossRefGoogle Scholar
  49. Samuelsen OB, Torsvik V, Ervik A (1992) Long-range changes in oxytetracycline concentration and bacterial resistance toward oxytetracycline in a fish farm sediment after medication. Sci Total Environ 114:25–36CrossRefGoogle Scholar
  50. Schuetze A, Heberer T, Juergensen S (2008a) Occurrence of residues of the veterinary drug malachite green in eels caught downstream from municipal sewage treatment plants. Chemosphere 72:1664–1670CrossRefGoogle Scholar
  51. Schuetze A, Heberer T, Juergensen S (2008b) Occurrence of residues of the veterinary drug crystal (Gentian) violet in wildlife eels caught downstream from municipal sewage treatment plants. Environ Chem 5:194–199CrossRefGoogle Scholar
  52. Schulte-Oehlmann U, Oetken M, Bachmann J, Oehlmann J (2004) In: Kümmerer K (ed) Pharmaceuticals in the environment. Sources, fate, effects and risks, 2nd edn, Springer-Heidelberg Berlin, pp 233–247Google Scholar
  53. Seki M, Yokota H, Matsubara H, Maeda M, Tadokoro H, Kobayashi K (2004) Fish full life-cycle testing for androgen methyltestosterone on medaka (Oryzias latipes) Environ Toxicol Chem 23:774–781CrossRefGoogle Scholar
  54. Sharpe RL, MacLatchy DL, Courtenay SC, Van Der Kraak GJ (2004) Effects of a model androgen (methyl testosterone) and a model anti-androgen (cyproterone acetate) on reproductive endocrine endpoints in a short-term adult mummichog (Fundulus heteroclitus) bioassay. Aquat Toxicol 67:203–215CrossRefGoogle Scholar
  55. Schachte JH Jr (1974) A short term treatment of malachite green and formalin for the control of Ichthyophthirius multifiliis on channel catfish in holding tanks. Prog Fish-Cult 36:103–104CrossRefGoogle Scholar
  56. Srivastava GC, Srivastava RC (1978) A note on the potential applicability of malachite green oxalate in combating fish-mycoses. Mycopathologia 23:774–781Google Scholar
  57. Sudova E, Machova J, Svobodova Z, Vesely T (2007) Negative effects of malachite green and possibilities of its replacement in the treatment of fish eggs and fish: A review. Vet Med-Czech 52:527–539Google Scholar
  58. Thompson HC, Rushing LG, Gehring T, Lochmann R (1999) Persistence of Gentian violet and leucogentian violet in channel catfish (Ictalurus punctatus) muscle after water-borne exposure. J Chromatogr B 723:287–291CrossRefGoogle Scholar
  59. WHO (2006) Antimicrobial use in aquaculture and antimicrobial Resistance. Report of a joint FAO/OIE/WHO expert consultation on antimicrobial use in aquaculture and antimicrobial resistance, Seoul, Republic of Korea, 13–16 June 2006, Geneva, pp 107Google Scholar
  60. Zhang X (2005) Hong Kong people fear poisonous Chinese fish. The Epoch Times,, Accessed 06 May 2008

Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  1. 1.Oldenburg Food InstituteLower Saxony Federal State Office of Consumer Protection and Food SafetyOldenburgGermany

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