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How Proteins Slide on DNA

  • Daniel Barsky
  • Ted A. Laurence
  • Česlovas Venclovas
Chapter
Part of the Biological and Medical Physics, Biomedical Engineering book series (BIOMEDICAL)

Abstract

Protein–DNA interactions are required for all the major functions of DNA: ­transcription and regulation, replication and repair, even the packaging of DNA into chromosomes. Not only are protein–DNA interactions crucial for all these cellular activities, but they are also, in our view, among the most fascinating macromolecular­ interactions because of their dynamics. In this chapter, we focus on DNA sliding by proteins, particularly diffusive sliding. Such sliding is typically part of the search for a target on the DNA itself or for another protein bound to the DNA. Of particular interest here are the proteins known as DNA sliding clamps that can remain bound to the DNA while diffusing vast distances along the double helix of DNA. We do not yet know the detailed mechanisms of protein sliding on DNA, but we aim to familiarize the reader with what is known observationally and to provide some discussion of potential mechanisms.

Keywords

Fluorescence Resonance Energy Transfer Diffusion Constant Total Internal Reflection Fluorescence lacI Repressor Fluorescence Resonance Energy Transfer Efficiency 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Daniel Barsky
    • 1
  • Ted A. Laurence
  • Česlovas Venclovas
  1. 1.Physical and Life Sciences Directorate, Lawrence Livermore National LaboratoryLivermoreUSA

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