Gene annotation used to refer to the prediction and annotation of a coding transcript on a region of the genome, but as the complexity of the functional features on the genome increases, users require prediction of noncoding RNAs, alternatively spliced transcripts, pseudogenes, and conserved elements. Eight years after the initial draft sequence of the human genome was published, the exact number of coding genes present on this sequence is still unclear. Since new sequencing technologies have reduced the cost of sequencing and dramatically increased the speed, we can expect an enormous expansion in the amount of available genomic and transcript sequence data. To gain insight into the functional information contained within these new sequences, the features within the sequence need to be accurately annotated.
KeywordsAlternative Splice Gene Annotation Manual Annotation Wellcome Trust Sanger Institute Distribute Annotation System
This work was supported by the Wellcome Trust.
- Christie KR, Weng S, Balakrishnan R, Costanzo MC, Dolinski K, Dwight SS et al (2004) Saccharomyces Genome Database (SGD) provides tools to identify and analyze sequences from Saccharomyces cerevisiae and related sequences from other organisms. Nucleic Acids Res 32(Database issue):D311–4CrossRefPubMedGoogle Scholar
- Donlin MJ (2007) Using the Generic Genome Browser (GBrowse). Curr Protoc Bioinformatics Chapter 9: Unit 9.9Google Scholar
- Misra S, Harris N (2006) Using Apollo to browse and edit genome annotations. Curr Protoc Bioinformatics Chapter 9: Unit 9.5Google Scholar