Abstract
Horseshoe crab populations along the northeast coast of India are under threat due to degradation of the breeding beaches. To augment the trend, attempts were made to culture the larvae of Tachypleus gigas and study its growth rate by enhancing the molting pattern in the laboratory condition. Trilobites of T. gigas were cultured on a controlled diet of brine shrimp (Artemia) and diatom (Chaetoceros gracilis) at 26–28°C and 32–34‰.
Trilobites could molt up to the fourth posthatched juvenile stage within a period of 180 days from the day of hatching of trilobite from the egg membrane as free swimming larval stage. The molting behavior was faster from the first to the third posthatched juvenile stage, i.e., within a period of 90 days. The average growth rate in terms of total body length from the first to second posthatched juvenile was about 63%, and from the second to third posthatched larva was about 38%. The growth rate was found to be about 25% from the third to fourth posthatched juvenile stage, and molting took place 180 days after the day of hatching of trilobite. All the posthatched juveniles had similar morphological features to the adults. The fourth posthatched juveniles exhibited more prominent morphological features with fully grown legs, spines, and segmentation, with a total body length of 45 mm.
Further studies on food-dependent molting patterns of juvenile instars may help to establish a standardized aquaculture method to grow horseshoe crabs in captivity. Sea ranching of these reared animals can be carried out regularly in the holding areas to increase horseshoe crab populations and conserve these precious organisms from the brink of extinction.
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References
Berkson J, Shuster C (1999) The horseshoe crab: the battle for a true multiple-use resource. Fisheries 24:6–10
Botton ML (2001) The conservation of horseshoe crabs: what can we learn from the Japanese experience? In: Tanacredi J (ed) Limulus in the Limelight. Kluwer Academic/Plenum, New York, pp 47–51
Botton ML, Loveland, RE, Tanacredi JT, Itow T (2006) Horseshoe crabs (Limulus polyphemus) in an urban estuary (Jamaica Bay, New York) and the potential for ecological restoration. Estuar Coasts 29: 820–830
Brown GG, Clapper DL (1981) Procedures for maintaining adults, collecting gametes, and culturing embryos and juveniles of the horseshoe crab, Limulus polyphemus L. In: Hinegardner R (ed) Laboratory Animal Management of Marine Invertebrates. National Academy Press, Washington, DC, pp 268–290
Carmichael RH, Rutecki D, Annett B, Gaines E, Valiela I (2004) Position of horseshoe crabs in estuarine food webs: N and C stable isotopic study of foraging ranges and diet composition. J Exp Mar Biol Ecol 299:231–253
Chatterji A, Vijaykumar, R, Parulekar AH (1988) Growth and morphometric characteristic in the horseshoe crab, Carcinoscorpius rotundicauda (Latreiile) from Canning (West Bengal), India. Pakistan J Sci Ind Res 33:352–355
Chatterji A, Kotnala S, Mathew R (2004) Effect of salinity on larval growth of horseshoe crab, Tachypleus gigas (Muller). Curr Sci 87:248–250
French KA (1979) Laboratory culture of embryonic and juvenile Limulus. In: Cohen E (ed), Biomedical Applications of the Horseshoe crab (Limulidae). Alan R Liss, New York, pp 61–71
Itow T, Mishra JK, Ahmed ATA (2004) Horseshoe crabs (King crabs) in the Bay of Bengal, South Asia. Bull Fac Edu Shizuoka Univ Nat Sci 54:13–30
Jegla TC (1982) A review of the molting physiology of the trilobite larva of Limulus. In: Bonaventura J, Bonaventura C, Tesh S (eds), Physiology and Biology of Horseshoe Crabs. Alan R Liss, New York, pp 83–101
Laughlin R (1983) The effects of temperature and salinity on larval growth of the horseshoe crab Limulus polyphemus. Biol Bull 164:93–103
Lee CN, Morton B (2005) Experimentally derived estimates of growth by juvenile, Tachypleus tridentatus and Carcinoscorpius rotundicauda (Xiphosura) from nursery beaches in Hong Kong. J Exp Mar Biol Ecol 318:39–49
Mishra JK (1991) Embryonic development and variations in the haemolymph of Indian horseshoe crab, Tachypleus gigas (Muller). Ph. D. Thesis, Berhampur University, Orissa, India
Mishra JK, Chatterji A, Parulekar AH (1992) A freak twin trilobite lava of the Indian horseshoe crab, Tachypleus gigas (Muller). J Bombay Nat Hist Soc 90:115–117
Mishra JK (1994) Horseshoe crab, an Amazing Creature of Orissa Coast. Project Swarajya Publ, Orissa, India
Rudloe A (1982) Man’s influence as an environmental threat to Limulus. In: Bonaventura J, Bonaventura C, Tesh S (eds), Physiology and Biology of Horseshoe Crabs. Alan R Liss, New York, pp 297–300
Satuito CG, Shimizu K, Natoyama K Yamazaki M, Fusetani N (1996) Age related settlement success by cyprids of the barnacle Balanus amphitrite, with special reference to consumption of cyprid storage protein. Mar Biol 127:125–130
Sekiguchi K (1973) A normal plate of the development of the Japanese horseshoe crab, Tachypleus tridentatus. Sci Rep Tokyo Kyoiku Daigaku Sect B 14:121–128
Sekiguchi K, Nakamura K, Sen TK, Sugita H (1976) Morphological variation and distribution of a horseshoe crab, Tachypleus gigas, from the Bay of Bengal and the Gulf of Siam. Proc Jap Soc Syst Zool 15:13–20
Sekiguchi K, Nakamura K, Seshimo H (1978) Morphological variation of horseshoe crab from the Bay of Bengal and gulf of Siam. Proc Jap Soc Syst Zool 15:24–30
Sekiguchi K (1988) Biology of Horseshoe Crabs. Science House, Tokyo
Sekiguch K, Itow T, Yamamich Y (1988) Experimental embryology. In: Sekiguchi K (ed). Biology of Horseshoe Crabs. Science House, Tokyo, pp 225–287
Sekiguchi K, Seshimo H, Sugita H (1988) Post-embryonic development of the horseshoe crab. Biol Bull 174:337–345
Shuster CN Jr, Sekiguchi K (2003) Growing up takes about ten years and eighteen stages. In: Shuster CN Jr, Barlow RB, Brockmann HJ (eds) The American Horseshoe Crab. Harvard Press, Cambridge, pp 103–132
Tanacredi JT (2001) Horseshoe crabs imperiled? The fate of a species 350 million years in the making. In: Tanacredi J (ed) Limulus in the Limelight. Kluwer Academic/Plenum, New York, pp 7–14
Widener JW, Barlow RB (1999) Decline of a horseshoe crab population on Cape Cod. Biol Bull 197:300–302
Acknowledgments
The author acknowledges the Ocean Science and Technology Cell, Andhra University, Visakhapatnam, India for providing laboratory facilities to carry out this research work. Thanks are due to two referees for their critical comments in improving the manuscript.
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Mishra, J.K. (2009). Larval Culture of Tachypleus gigas and Its Molting Behavior Under Laboratory Conditions. In: Tanacredi, J., Botton, M., Smith, D. (eds) Biology and Conservation of Horseshoe Crabs. Springer, Boston, MA. https://doi.org/10.1007/978-0-387-89959-6_32
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DOI: https://doi.org/10.1007/978-0-387-89959-6_32
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