The following comprises a technical disclosure of the reagents and methods used in preparing the cytology and cell block histology slides of endometrial brushings. The methods are based on cumulative experience with processing liquid-based cytology without specialized preparative instruments.
The reagents and methods disclosed herein have evolved over several years and are generally applicable to cytopathology. We have tested them with more than 125,000 cervicovaginal specimens, 10,000 endometrial specimens, and 25,000 nongynecological specimens, including brushings and fine-needle aspiration biopsies. The same methods may be applied to fluid cytology after the naturally occurring cell suspension has been concentrated to a cell button, decanted, and fixed.
KeywordsCell Block Liquid Circle Fluid Cytology Room Temperature Water Cell Button
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