Array Comparative Genomic Hybridization in Pathology
Comparative genomic hybridization (CGH) is a molecular cytogenetic method for the detection and mapping of chromosomal gains and losses.1 It is based on the cohybridization of differentially labeled test and reference DNA onto metaphase spreads, which usually have been prepared from peripheral blood lymphocytes of a healthy donor. The signal intensity ratios of the two labels along the chromosomes then reflect DNA copy number changes in the test genome relative to the reference genome. Although CGH has tremendously contributed to our knowledge of chromosomal aberrations, its resolution, unfortunately, is limited to about 3–10 Mb.2 Resolution of CGH has significantly improved when samples were no longer hybridized to metaphase spreads but to DNA targets that have been arrayed on a glass substrate. This modification to the original technique has been named array CGH3 or matrix CGH,4 respectively. In theory, resolution of array CGH is only limited by the number and quality of DNA targets arrayed on the slide. The principle of array CGH is illustrated in Fig. 10.1.
KeywordsBacterial Artificial Chromosome Comparative Genomic Hybridization Array Comparative Genomic Hybridization Whole Genome Amplification Array Comparative Genomic Hybridization Analysis
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