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Transcript Slippage and Recoding

  • Michael Anikin
  • Vadim Molodtsov
  • Dmitry Temiakov
  • William T. McAllister
Chapter
Part of the Nucleic Acids and Molecular Biology book series (NUCLEIC, volume 24)

Abstract

Accurate transmission of genetic information during transcription requires that RNA polymerases maintain the correct register of the active site during each cycle of nucleotide incorporation. The RNA:DNA hybrid plays an important role in maintaining this lateral stability, and it has been observed that when the polymerase encounters homopolymeric tracts in the DNA template the transcript and/or the transcription complex may slip along the template, allowing the polymerase to incorporate more or fewer nucleotides than are encoded by the template. This phenomenon has been observed during all phases in the transcription cycle, including initiation, elongation, and termination. Here we review the evidence for transcript slippage in vivo and its implications for miscoding events. In addition, we review experiments that bear upon the mechanistic aspects of transcript slippage and the parameters that may affect its frequency. Aside from its implications for miscoding, transcript slippage may also be involved in regulatory roles during initiation and termination and promote expression of alternative information from the same gene.

Keywords

Elongation Complex Nucleotide Incorporation Homopolymeric Tract Brownian Ratchet Transcription Bubble 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

These studies were supported by grants from the National Institutes of Health (GM38147) and from the Foundation of UMDNJ to WTM. We are grateful to Chuck Turnbough, Don Luse, Sergei Borukhov, Dimitriy Markov, Steven Emanuel, and Maria Savkina for helpful comments, and to Mr. Raymond Castagna for technical support. We thank Craig Martin for pointing out to us the special properties of An:Tn homoduplexes that might provide a basis for transcript slippage, and Irina Artsimovitch and Evgeny Nudler for the gift of EcoRIQ111A.

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Michael Anikin
    • 1
  • Vadim Molodtsov
    • 1
  • Dmitry Temiakov
    • 1
  • William T. McAllister
    • 2
  1. 1.Department of Cell Biology, School of Osteopathic MedicineUniversity of Medicine and Dentistry of New JerseyStratfordUSA
  2. 2.Department of Cell Biology, School of Osteopathic MedicineUniversity of Medicine and Dentistry of New JerseyStratfordUSA

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