Translational Bypassing – Peptidyl-tRNA Re-pairing at Non-overlapping Sites

Part of the Nucleic Acids and Molecular Biology book series (NUCLEIC, volume 24)


Ribosomal bypassing can lead to the translational fusion of non-contiguous ORFs. It involves dissociation of codon:anticodon pairing in the ribosomal P-site followed by mRNA slippage and re-pairing of the retained tRNA anticodon to mRNA at a non-overlapping codon. It is frame independent. The most studied case involves the bypassing of 50 non-coding nucleotides between codons 46 and 47 of phage T4 gene 60 where half the translating ribosomes successfully accomplish the feat. A nascent peptide signal encoded 5 of the start of the coding gap facilitates the initial codon:anticodon dissociation. An mRNA structure forms in the ribosomal A-site. Only when sequence participating in this structure has passed the ribosomal P-site does the potential for anticodon re-pairing to mRNA at a matched codon arise. After such re-pairing, normal decoding of the A-site codon mediates resumption of standard translation.


Landing Site Stem Loop Codon Pairing mRNA Structure Nascent Peptide 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This work was supported by NIH grant ROI GM079523.


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© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  1. 1.Department of Human GeneticsUniversity of UtahSalt Lake CityUSA

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