Veterinary Drugs and Growth Promoters Residues in Meat and Processed Meats

  • Milagro Reig
  • Fidel Toldrá
Part of the Food Microbiology and Food Safety book series (FMFS)


Veterinary drugs, which comprise a large number of different types of substances, are generally intended for therapeutic (to control infectious diseases) and prophylactic (to prevent against infections) purposes in farm animals. Other substances with growth promoting effect may exert antimicrobial effect against the microbial flora in the gut to take maximum profit of nutrients in the feed or by affecting the animal’s metabolism. Most of these substances are orally active and can be administered either in the feed or in the drinking water. Other active hormones are applied in the form of small implants into the subcutaneous tissue of the ears. These are slow release (several weeks or months) devices and the ears are discarded at the slaughter. Growth promoters allow a better efficiency in the feed conversion rate. The net effect is an increased protein deposition, partly due to muscle proteases inhibition (Fiems, Buts, Boucque, Demeyer, & Cottyn, 1990), usually linked to...


Growth Promoter Anabolic Effect Maximum Residue Limit Veterinary Drug Macrocyclic Lactone 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



Project A-05/08 from Conselleria de Sanitat, Generalitat Valenciana (Valencia, Spain) is acknowledged.


  1. Barbosa, J., Cruz, C., Connolly, L., Elliott, C. T., Lovgren, T., & Tuomola, M. (2005). Food poisoning by clenbuterol in Portugal. Food Additives and Contaminants, 22, 563–566.CrossRefGoogle Scholar
  2. Bergweff, A. A., & Schloesser, J. (2003). Residue determination. In B. Caballero, L. Trugo, & P. Finglas (Eds.), Encyclopedia of food sciences and nutrition (2nd ed., pp 254–261). London, UK: Elsevier.CrossRefGoogle Scholar
  3. Bergweff, A. A. (2005). Rapid assays for detection of residues of veterinary drugs. In A. van Amerongen, D. Barug, & M. Lauwars (Eds.), Rapid methods for biological and chemical contaminants in food and feed (pp. 259–292). The Netherlands: Wageningen Academic Publishers.Google Scholar
  4. Brockman, R. P., & Laarveld, R. (1986). Hormonal regulation of metabolism in ruminants. Review. Livestock Production Science, 14, 313–317.CrossRefGoogle Scholar
  5. Byrnes, S. D. (2005). Demystifying 21 CFR Part 556—Tolerances for residues of new animal drugs in food. Regulatory Toxicology and Pharmacology, 42, 324–327CrossRefGoogle Scholar
  6. Cerniglia, C. E., & Kotarski, S. (2005). Approaches in the safety evaluations of veterinary antimicrobial agents in food to determine the effects on the human intestinal microflora. Journal of Veterinary Pharmacology and Therapy, 28, 3–20.CrossRefGoogle Scholar
  7. Cerniglia, C. E., & Kotarski, S. (1998). Evaluation of veterinary drug residues in food for their potential to affect human intestinal microflora. Regulatory Toxicology and Pharmacology, 29, 238–261.CrossRefGoogle Scholar
  8. CFR (2008). Tolerances for residues of new animal drugs in food, Code of Federal Regulations, Title 21 Food and Drugs, Chapter I, subchapter E, Part 556, (accessed June 3, 2008),
  9. Chadwick, R. W., George, S. E., & Claxton, L. D. (1992). Role of gastrointestinal mucosa and microflora in the bioactivation of dietary and environmental mutagens or carcinogens. Drug Metabolism Reviews, 24, 425–492.CrossRefGoogle Scholar
  10. Croubels, S., Daeselaire, E., De Baere, S., De Backer, P., & Courtheyn, D. (2004). Feed and drug residues. In W. Jensen, C. Devine, & M. Dikemann (Eds.), Encyclopedia of meat sciences (pp. 1172–1187). London, UK: Elsevier.CrossRefGoogle Scholar
  11. Dixon (2001). Veterinary drug residues. In: Food Chemical safety, vol. 1: Contaminants (D.H. Watson, Ed.), Woodhead Pub. Ltd., Cambridge, UK, 109 –147.Google Scholar
  12. EC (2002). Commission Decision 2002/657/EEC of 17 August 2002 implementing Council Directive 96/23/EC concerning the performance of the analytical methods and the interpretation of results. Official Journal of the European Community L, 221, 8.Google Scholar
  13. EC (1993a). Commission Decision 93/256/EEC of 14 May 1993 laying down the methods to be used for detecting residues of substances having hormonal or a thyreostatic action. Official Journal of the European Community L, 118, 64.Google Scholar
  14. EC (1993b). Commission Decision 93/256/EEC of 15 April 1993 laying down the reference methods and the list of the national reference laboratories for detecting residues. Official Journal of European Community L, 118, 73.Google Scholar
  15. EC (1988). Council Directive 88/146/EEC of 7 March 1988 prohibiting the use in livestock farming of certain substances having a hormonal action. Official Journal of the European Community L, 070, 16.Google Scholar
  16. EC (1996). Council Directive 96/23/EEC of 29 April 1996 on measures to monitor certain substances and residues thereof in live animals and animal products. Official Journal of the European Community L, 125, 10.Google Scholar
  17. EFSA (2007). Opinion of the scientific panel on contaminants in the food chain on a request from the European Commission related to hormone residues in bovine meat and meat products. The EFSA Journal, 510, 1–62.Google Scholar
  18. Ellis, R. (2004). U.S.F.D.A. Regulatory Approach for Control of Residues of Veterinary Drugs, Joint FAO/WHO Technical Workshop on Residues of Veterinary without ADI/MRL, Bangkok, Thailand, 24–26 august 2004, 49–55.Google Scholar
  19. Fiems, L. O., Buts, B., Boucque, C. V., Demeyer, D. I., & Cottyn, B. G. (1990). Effect of a β-agonist on meat quality and myofibrillar protein fragmentation in bulls. Meat Science, 27, 29–35.CrossRefGoogle Scholar
  20. Guo, J. J., Chou, H. N., & Liao, I. C. (2003). Disposition of 3-(4-cyano-2-oxobutylidene amino)-2-oxazolidone, a cyano-metabolite of furazolidone, in furazolidone-treated grouper. Food Additives and Contaminants, 20, 229–236.CrossRefGoogle Scholar
  21. Hagren, V., Connolly, L., Elliott, C. T., Lovgren, T., & Tuomola, M. (2005). Rapid screening method for halofuginone residues in poultry eggs and liver using time-resolved fluorometry combined with the all-in-one dry chemistry assay concept. Analytica Chimica Acta, 529, 21–25.CrossRefGoogle Scholar
  22. Leffers, H., Naesby, M., Vendelbo, B., Skakkebaek, N. E., & Jorgensen, M. (2001). Oestrogenic potencies of zeranol, oestradiol, diethylstilboestrol, bisphenol A and genistein: implications for exposure assessment of potential endocrine disrupters. Human Reproductivity, 16, 1037–1045.CrossRefGoogle Scholar
  23. Lone, K. P. (1997). Natural sex steroids and their xenobiotic analogs in animal production: Growth, carcass quality, pharmacokinetics, metabolism, mode of action, residues, methods, and epidemiology. Critical Reviews in Food Science and Nutrition, 37, 93–209.CrossRefGoogle Scholar
  24. Miller, L. F., Judge, M. D., Diekman, M. A., Hudgens, R. E., & Aberle, E. D. (1989). Relationships among intramuscular collagen, serum hydroxyproline and serum testosterone in growing rams and wethers. Journal of Animal Science, 67, 698–703.Google Scholar
  25. Miller, L. F., Judge, M. D., & Schanbacher, B. D. (1990). Intramuscular collagen and serum hydroxyproline as related to implanted testosterone and estradiol 17β in growing wethers. Journal of Animal Science, 68, 1044–1048.Google Scholar
  26. Monsón, F., Sañudo, C., Bianchi, G., Albertí, P., Herrera, A., & Ariño, A. (2007). Carcass and meat quality of yearling bulls as affected by the use of clenbuterol and steroid hormones combined with dexamethasone. Journal of Muscle Foods, 18, 173–185.CrossRefGoogle Scholar
  27. Moore, W. E. C., & Moore, L. H. (1995). Intestinal floras of populations that have a high risk of colon cancer. Applied and Environmental Microbiology, 61, 3202–3207.Google Scholar
  28. Mottier, P., Parisod, V., Gremaud, E., Guy, P. A., & Stadler, R. H. (2003). Determination of the antibiotic chloramphenicol in meat and seafood products by liquid chromatography–electrospray ionization tandem mass spectrometry. Journal of Chromatography A, 994, 75–84.CrossRefGoogle Scholar
  29. Pecorelli, I., Bibi, R., Fioroni, L., & Galarini, R. (2004). Validation of a confirmatory method for the determination of sulphonamides in muscle according to the European Union regulation 2002/657/EC. Journal of Chromatography A, 1032, 23–29.CrossRefGoogle Scholar
  30. Peippo, P., Lovgren, T., & Tuomola, M. (2005). Rapid screening of narasin residues in poultry plasma by time-resolved fluoroimmunoassay. Analytica Chimica Acta, 529, 27–31.CrossRefGoogle Scholar
  31. Perry, G. A., Welshons, W. V., Bott, R. C., & Smith, M. F. (2005). Basis of melengestrol acetate action as a progestin. Domestic Animal Endocrinology, 28, 147–161.CrossRefGoogle Scholar
  32. Reig, M., & Toldrá, F. (2007). Chemical origin toxic compounds. In F. Toldrá, Y. H. Hui, I. Astiasarán, W. K. Nip, J. G. Sebranek, E. T. F. Silveira, L. H. Stahnke, & R. Talon (Eds.), Handbook of fermented meat and poultry (pp. 469–475). Ames, Iowa, USA: Blackwell Publishing.Google Scholar
  33. Reig, M., & Toldrá, F. (2008). Veterinary drug residues in meat: Concerns and rapid methods for detection. Meat Science, 78, 60–67.CrossRefGoogle Scholar
  34. Reig, M., & Toldrá, F. (2009a). Growth promoters. In L. M. L. Nollet, & F. Toldrá (Eds.), Handbook of muscle foods analysis (pp. 837–854). Boca Raton, FL: CRC Press.Google Scholar
  35. Reig, M., & Toldrá, F. (2009b). Veterinary drug residues. In L. M. L. Nollet, & F. Toldrá (Eds.), Handbook of meat products analysis (pp. 637–653). Boca Raton, FL: CRC Press.Google Scholar
  36. Takemura, H., Shim, J. Y., Sayama, K., Tsubura, A., Zhu, B. T., & Shimoi, K. (2007). Characterization of the estrogenic activities of zearalenone and zeranol in vivo and in vitro. Journal of Steroid Biochemistry and Molecular Biology, 103, 170–177.CrossRefGoogle Scholar
  37. Toldrá, F., & Reig, M. (2006). Methods for rapid detection of chemical and veterinary drug residues in animal foods. Trends in Food Science and Technology, 17, 482–489.CrossRefGoogle Scholar
  38. van den Bogaard, A. E., Bruinsma, N., & Stobberingh, E. E. (2000). The effect of banning avoparcin on VRE carriage in The Netherlands. Journal of Antimicrobial Chemotherapy, 46, 146–148.CrossRefGoogle Scholar
  39. Van Peteguem, C., & Daeselaire, E. (2004). Residues of Growth Promoters. In L. M. L. Nollet (Ed.), Handbook of food analysis (2nd ed., pp. 1037–1063). New York: Marcel Dekker Inc.Google Scholar
  40. Verdon, E. (2009). Antibiotic residues in muscle tissues of edible animal products. In L. M. L. Nollet, & F. Toldrá (Eds.), Handbook of meat products analysis (pp. 856–947). Boca Raton, FL: CRC Press.Google Scholar
  41. Vollard, E. J., & Clasener, H. A. L. (1994). Colonization resistance. Antimicrobial Agents and Chemotherapy, 38, 409–414.Google Scholar
  42. Wilson, V. S., Lambright, C., Ostby, J., & Gray, L. E., Jr. (2002). In vitro and in vivo effects of 17 beta-trenbolone: a feedlot effluent contaminant. Toxicological Science, 70, 202–211.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  1. 1.Institute of Engineering for Food DevelopmentPolytechnical University of ValenciaSpain
  2. 2.Department of Food ScienceInstituto de Agroquímica y Tecnología de Alimentos (CSIC)Spain

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