Municipal Wastewater Concentrations of Pharmaceutical and Xeno-Estrogens: Wildlife and Human Health Implications

  • Maxine Wright-Walters
  • Conrad Volz
Conference paper


Most pharmaceutical estrogens and xenoestrogens are introduced into the environment through municipal waste water treatment plant (WWTP) effluent sources. These effluents contain synthetic compounds; surfactants, flame retardants and halogenated hydrocarbons that can mimic estrogens; and are discharged directly into rivers and lakes. As rivers and lakes are used for water and food supply, and recreation, and wastewater effluent usage increases, the presence and concentration of xenoestrogens in surface water becomes a valid public health concern. Additionally, many USA cities have significant combined sewer overflows releasing untreated sewage directly into surface waters, thus increasing the amounts of xenoestrogens finding their way into drinking water supplies and commercial and subsistence fishing habitat.

In the United States, humans are exposed daily to both pharmaceutical and xenoestrogens which have been implicated in various human health outcomes, such as testicular dysgenesis syndrome including testicular cancer and breast cancer in women. Also, they can have adverse reproductive effects in aquatic wildlife through sex reversals, production of intersex individuals, alterations in mating, and prevention of gonadal maturation. Combinations of estrogenic compounds are present in municipal WWTP effluents but, the natural estrogens, 17β-estradiol (E2) and estrone (E1), and the synthetic E2 derivate 17α-ethinylestradiol (EE2) are most responsible for in vitro estrogenic activity. Each xenoestrogen exhibits its own wildlife or human health risk, but synergistic effects could occur with xenoestrogen mixtures. Less than 1 ng/L EE2 can cause feminization of male fishes, 4 ng/L caused abnormal reproductive development (male fathead minnows). E2 has been detected at concentrations from 1 ng/L to 80 ng/L. Total estrogenicity (E2 equivalents) of 147 ng/L has been measured in WWTP effluent. Nonylphenol, a surfactant and brominated biphenyls, a flame retardant have been detected between 0.1–3.7 μg/L and 0.3–4.6 mg/kg (on suspended particles) respectively.

Understanding the species and xenoestrogen concentrations in surface water is imperative for environmental public health tracking of associated disease states. Such research will determine the necessity for utilizing limited and competing public financial resources to invest in technology to remove xenoestrogens from surface waters and, in regulation of fish or wildlife consumption from our rivers and lakes.


Water Through Municipal Wastewater Treatment Plant Municipal WWTPs Sewage Treatment Work Diethyl Hexyl Phthalate Testicular Dysgenesis Syndrome 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. Adlercreutz H. (1995). “Phytoestrogens: epidemiology and a possible role in cancer protection." Environ Health Perspect 7 (103): 103–112.CrossRefGoogle Scholar
  2. Atienzar FA, Billinghurst Z, Depledge MH. (2002). “4-n-Nonylphenol and 17-beta estradiol may induce common DNA effects in developing barnacle larvae." Environ Pollut 3 (120): 735–738.CrossRefGoogle Scholar
  3. Banerjee SK, Banerjee S, Li SA, Li JJ. (1994). “Induction of chromosome-aberrations in Syrian-hamster renal cortical-cells by various estrogens." Mutat Res Fundam Mol Mech Mutagen 2 (311): 191–197.Google Scholar
  4. Baronti C, Curini R, D’Ascenzo G, Di Corcia A, Gentili A, Saperi R. (2000). “Monitoring natural and synthetic estrogens at activated sludge sewage treatment plants and in a receiving river water." Environ Sci Technol 34: 5059.CrossRefGoogle Scholar
  5. Belfroid A, Van der Horst A, Vethaak AD, Schäfer AJ, Rijs GBJ, Wegener J, Confino WP. (1999). Sci Total Environ 225: 101–108.CrossRefGoogle Scholar
  6. Bolz U, Hagenmaier H, et al. (2001). “Phenolic xenoestrogens in surface water, sediments, and sewage sludge from Baden-Wurttemberg, south-west Germany." Environ Pollut 115 (2): 291–301.CrossRefGoogle Scholar
  7. Carlsen E, Giwercman A, Keiding N, Skakkebaek NK. (1995). “Declining semen quality and increasing incidence of testicular cancer: Is there a common cause?" Environ Health Perspect 103: 137–139.CrossRefGoogle Scholar
  8. Choi SM, Yoo SD, Lee BM. (2004). “Toxicological characteristics of endocrine-disrupting chemicals: developmental toxicity, carcinogenicity, and mutagenicity." J Toxicol Environ Health B Crit Rev 1 (7): 1–32.Google Scholar
  9. Colborn T, vom Saal SF, Soto AM. (1993). “Developmental effects of endocrine-disrupting chemicals in wildlife and humans." Environ Health Perspect 101: 378–384.CrossRefGoogle Scholar
  10. Crain DA, Spiteri I, Guillette LJ Jr. (1999). “The functional and structural observations of the neonatal reproductive system of alligators exposed in ovo to atrazine, 2,4-D or estradiol." Toxicol Ind Health 15: 180–185.CrossRefGoogle Scholar
  11. Daughton CG, Ternes TA. (1999). “Pharmaceuticals and Personal Care Products in the Environment: Agents of Subtle Change?" Environ Health Perspect 107 (Suppl 6): 907–938.CrossRefGoogle Scholar
  12. Desbrow C, Routledge EJ, Brighty GC, Sumpter JP, Waldock M. (1998). “Identification of estrogenic chemicals in STW effluent. 1. Chemical fractionation and in vitro biological screening." Environ Sci Technol 11 (32): 1549–1558.CrossRefGoogle Scholar
  13. Feyk LA, Giesy JP. (1998). “Xenobiotic modulation of endocrine function in birds. In: Principles and Processes for Evaluating Endocrine Disruption in Wildlife (Kendall R, Dickerson R, Giesy J, Suk W, eds)." Pensacola, FL, SETAC Press, pp. 121–140.Google Scholar
  14. Gaido KW, Leonar L, Lovell S, Gould JC, Babai D, Portier CJ, et al. (1997). “Evaluation of chemicals with endocrine modulating activity in a yeast-based steroid hormone receptor gene transcription assay." Toxicol Appl Pharmacol 1 (143): 205–212.CrossRefGoogle Scholar
  15. Giesy JP, Ludwig JP, Tillitt DE. (1994). “Deformities of birds in the Great Lakes region: assigning causality." Environ Sci Technol 28: 128A–135A.CrossRefGoogle Scholar
  16. Gimeno, S. G., A. Bowmer, T. Komen, H. (1996). “Feminization of male carp” Nature 384(6606): 221–222Google Scholar
  17. Giwercman A, Carlsen E, Keiding N, Skakkebaek NE. (1993). “Evidence for increasing incidence of abnormalities of the human testis: A review." Environ Health Perspect 101: 65–72.CrossRefGoogle Scholar
  18. Gray LE, Ostby JS, Kelce WR. (1994). “Developmental effects of an environmental antiandrogen: the fungicide vinclozolin alters differentiation of the male rat." Toxicol Appl Pharmacol 129: 46–52.CrossRefGoogle Scholar
  19. Gray, M.A., and Metcalfe, Chris D. (1997). “Induction of Testis–Ova in Japanese Medaka (Oryzias Latipes) Exposed to p-Nonylphenol.” Environmental Toxicology and Chemistry 1082–1086.Google Scholar
  20. Guillette LJ Jr, Crain DA, Rooney AA, Pickford DB. (1995). “Organization versus activation: the role of endocrine-disrupting contaminants (EDCs) during embryonic development in wildlife." Environ Health Perspect 103 (Suppl 7): 157–164.CrossRefGoogle Scholar
  21. Guillette LJ Jr, Gross TS, Masson GR, Matter JM, Percival HF, Woodward AR. (1994). “Developmental abnormalities of the gonad and abnormal sex hormone concentrations in juvenile alligators from contaminated and control lakes in Florida." Environ Health Perspect 102: 680–688CrossRefGoogle Scholar
  22. Guillette LJ Jr, Pickford DB, Crain DA, Rooney AA, Percival HF. (1996). “Reduction in penis size and plasma testosterone concentrations in juvenile alligators living in a contaminated environment." Gen Comp Endocrinol 101: 32–42.CrossRefGoogle Scholar
  23. Han XL, Liehr JG. (1994). “8-Hydroxylation of guanine bases in kidney and liver DNA of hamsters treated with estradiol-role of free radicals in estrogen-induced carcinogenesis." Cancer Res 21 (54): 5515–5517.Google Scholar
  24. Harris CA, Santos EM, Janbakhsh A, Pottinger TG, Tyler CR, Sumpter JP. (2001). “Nonylphenol affects gonadotropin levels in the pituitary gland and plasma of female rainbow trout." Environ Sci Technol 14 (35): 2909–2916.CrossRefGoogle Scholar
  25. Hayes TB, Collins A, Lee M, Mendoza M, Noriega N, Stuart AA, et al. (2002). “Hermaphroditic, demasculinized frogs after exposure to the herbicide atrazine at low ecologically relevant doses." Proc Natl Acad Sci USA 99: 5476–5480.CrossRefGoogle Scholar
  26. Hutson SS, Barber NL, Kenny JF, Linsey KS, Lumia DS, Maupin MA. (2004). Estimated Use of Water in the United States in 2000. USGS. U.S. Department of Interior, U.S. Geological Survey. U.S. Geological Survey Circular 1268. Google Scholar
  27. Hyötyläinen T, Grob K, Biedermann M, Riekkola ML. (1997). “Reversed phase HPLC coupled on-line to GC by the vaporizer/precolumn solvent split/gas discharge interface; analysis of phthalates in water." J High Resolut Chromatogr 20: 410.CrossRefGoogle Scholar
  28. Jin XL, Huang GL, et al. (2004). “Simultaneous determination of 4-tert-octylphenol, 4-nonylphenol and bisphenol a in Guanting Reservoir using gas chromatography-mass spectrometry with selected ion monitoring." J Environ Sci (China) 16 (5): 825–828.Google Scholar
  29. Jobling S, Tyler CR. (2003). “Endocrine Disruption in Wild Freshwater Fish." Pure Appl Chem 75 (11–12): 2219–2234.CrossRefGoogle Scholar
  30. Jobling S, Nolan M, Tyler CR, Brighty G, Sumpter JP. (1998). “Widespread sexual disruption in wild fish." Environ Sci Technol 17 (32): 2498–2506.CrossRefGoogle Scholar
  31. Kidd KA, Blanchfield PJ, et al. (2007). “Collapse of a fish population after exposure to a synthetic estrogen." Proc Natl Acad Sci USA 104 (21): 8897–8901.CrossRefGoogle Scholar
  32. King TE, Ballereau SJ, et al. (2006). “Genetic signatures of coancestry within surnames." Curr Biol 16 (4): 384–388.CrossRefGoogle Scholar
  33. Körner W, Bolz U, Rita T, Schwaiger J, Rolf-Dieter N, Hagenmaier AMaH. (2001). “Steroid Analysis and Xenosteroid Potentials in the Small Streams in Southwest Germany." J Aquat Ecosyst Stress and Recovery 8: 215–229.Google Scholar
  34. Kuch HM, Ballschmiter K. (2001). “Determination of endocrine-disrupting phenolic compounds and estrogens in surface and drinking water by HRGC-(NCI)-MS in the picogram per liter range." Environ Sci Technol 35 (15): 3201–3206.CrossRefGoogle Scholar
  35. Larsson D, Adolfsson-Erici M, Parkkonen J, Pettersson M, Berg AH, Olsson P-E, Forlin L. (1999). Aquat Toxicol (45): 91–97.Google Scholar
  36. Liney KE, Hagger JA, et al. (2006). “Health effects in fish of long-term exposure to effluents from wastewater treatment works." Environ Health Perspect 114 (Suppl 1): 81–89.Google Scholar
  37. Magliulo L, Schreibman M, Cepriano J, Ling J. (2002). “Endocrine disruption caused by two common pollutants at “acceptable” concentrations." Neurotoxicol Teratol 1 (24): 71–79.CrossRefGoogle Scholar
  38. Meyer VF. (2001). “The Medicalization of Menopause: Critique and Consequences." Int J Health Sci 4 (31): 769–792.CrossRefGoogle Scholar
  39. Norris DO. (2007). Xenoestrogen Actions on Reproduction: Implications for Health of Wildlife And Humans. American Water Resources Association 2007 Summer Specialty Conference, Emerging Contaminants of Concern in the Environment: Issues, Investigations and Solutions.Google Scholar
  40. Nutter LM, Ngo EO, Abulhajj YJ. (1991). “Characterization of DNA damage induced by 3,4-estrone-ortho-quinone in human-cells." J Biol Chem 25 (266): 16380–16386.Google Scholar
  41. Nutter LM, Wu YY, Ngo EO, Sierra EE, Gutierrez PL, Abulhajj YJ. (1994). “An O-quinone form of estrogen produces free radicals in human breast-cancer cells-correlation with DNA-damage." Chem Res Toxicol 1 (7): 23–28.CrossRefGoogle Scholar
  42. Petrovic M, Barcelo D. (2000). “Determination of Anionic and Nonionic Surfactants, Their Degradation Products, and Endocrine-Disrupting Compounds in Sewage Sludge by Liquid Chromatography/Mass Spectrometry." Anal Chem 72 (19): 4560–4567.CrossRefGoogle Scholar
  43. Purdom CE, Hardiman, PA, Bye VJ, Eno NC, Tyler CR, Sumpter JP. (1994). “Estrogenic effects of effluents from sewage treatment works.” Chem Ecol 8: 275–285.CrossRefGoogle Scholar
  44. Rajapakse N, Silva E. et al. (2002). “Combining xenoestrogens at levels below individual no-observed-effect concentrations dramatically enhances steroid hormone action." Environ Health Perspect 110 (9): 917–921.CrossRefGoogle Scholar
  45. Routledge EJ, Sheahan D, et al. (1998). “Identification of Estrogenic Chemicals in STW Effluent. 2. In Vivo Responses in Trout and Roach." Environ Sci Technol 32 (11): 1559–1565.CrossRefGoogle Scholar
  46. Safe SH. (2000). “Endocrine Disruptors and Human Health-Is There a Problem? An Update." Environ Health Perspect 108 (6): 487–493.Google Scholar
  47. Sharpe RM, Fisher JS, et al. (1995). “Gestational and lactational exposure of rats to xenoestrogens results in reduced testicular size and sperm production." Environ Health Perspect 103 (12): 1136–1143.CrossRefGoogle Scholar
  48. Silva E, Rajapakse N, Kortenkamp A. (2002). “Something from “nothing”-eight weak estrogenic chemicals combined at concentrations below NOECs produce significant mixture effects." Environ Sci Technol 8 (36): 1751–1756.CrossRefGoogle Scholar
  49. Sohoni P, Sumpter JP. (1998). “Several environmental oestrogens are also anti-androgens." J Endocrinol 158 (3): 327–339.CrossRefGoogle Scholar
  50. Stachel B, Ehrhorn U. et al. (2003). “Xenoestrogens in the River Elbe and its tributaries." Environ Pollut 124 (3): 497–507.CrossRefGoogle Scholar
  51. Stevens JL, Northcott GL, Stern GA, Tomy GT, Jones KC. (2003). “PAHs, PCBs, PCNs, organochlorine pesticides, synthetic musks, and polychlorinated n-alkanes in UK sewage sludge: survey results and implications." Environ Sci Technol 3 (37): 462–467.CrossRefGoogle Scholar
  52. Sumpter J. (2003). “Endocrine disruption in wildlife: the future?" Pure Appl Chem 11–12 (75): 2355–2360.CrossRefGoogle Scholar
  53. Sumpter JP, Jobling S. (1995). “Vitellogenesis as a biomarker for estrogenic contamination of the aquatic environment." Environ Health Perspect 103 (Suppl 7): 173–178.CrossRefGoogle Scholar
  54. Ternes TA, Bonerz M, et al. (2007). “Irrigation of treated wastewater in Braunschweig, Germany: an option to remove pharmaceuticals and musk fragrances." Chemosphere 66 (5): 894–904.CrossRefGoogle Scholar
  55. Ternes TA, Kreckel P, et al. (1999). “Behaviour and occurrence of estrogens in municipal sewage treatment plants – II. Aerobic batch experiments with activated sludge." Sci Total Environ 225 (1–2): 91–99.CrossRefGoogle Scholar
  56. Thorpe KL, Cummings RI, Hutchinson TH, Scholze M, Brighty G, Sumpter JP, et al. (2003). “Relative potencies and combination effects of steroidal estrogens in fish." Environ Sci Technol 6 (37): 1142–1149.CrossRefGoogle Scholar
  57. Thorpe KL, Hutchinson TH, Hetheridge MJ, Scholze M, Sumpter JP, Tyler CR. (2001). “Assessing the biological potency of binary mixtures of environmental estrogens using vitellogenin induction in juvenile rainbow trout (Oncorhynchus mykiss)." Environ Sci Technol 12 (35): 2476–2481.CrossRefGoogle Scholar
  58. Toppari J, Larsen JC, Christiansen P, Giwercman A, Grandjean P, Guillette LJ, Jr, Jégou B, Jensen TK, Jounnet P, Keiding N, et al. (1996). “Male reproductive health and environmental xenoestrogens." Environ Health Perspect Suppl 104: 741.CrossRefGoogle Scholar
  59. University of Michigan, C. f. S. S. (2005). U.S. Water Supply and Distribution.Google Scholar
  60. USEPA. (2001). Removal of Endocrine Disrupting Chemicals in Drinking Water. D. Office of Research and Development Washington, Technology Transfer and Support Division, National Risk Management Research Laboratory, Office of Research and Development, U.S. Environmental Protection Agency, Cincinnati, Ohio 45268.Google Scholar
  61. USEPA. (2004). Primer for Municipal Wastewater Treatment Systems. O. o. W. O. o. W. Management, Washington, DC.Google Scholar
  62. Willingham E, Crews D. (1999). “Sex reversal effects of environmentally relevant xenobiotic concentrations on the redeared slider turtle, a species with temperature-dependent sex determination.” Gen Comp Endocrinol 113: 429–435.CrossRefGoogle Scholar
  63. Zhou J, Liu R, Wilding A, Hibberd A. (2007). “Sorption of Selected Endocrine Disrupting Chemicals to Different Aquatic Colloids.” Environ Sci Technol 41: 206–213.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Maxine Wright-Walters
    • 1
  • Conrad Volz
    • 2
  1. 1.Department of Environmental and Occupational HealthGraduate School of Public Health, University of PittsburghPittsburghUSA
  2. 2.North Carolina Agricultural and Technical State UniversityGreensboroUSA

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