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Analyzing Gene Expression

  • Holly A. LaVoie
  • Pedro J. Chedrese
Chapter

Changes in activity of regulated genes correlate with the synthesis of the encoded proteins directly affecting the physiological status of the individual. In most of the cases, protein synthesis is directly proportional to the transcriptional activity of its gene, which affects the steady state levels of mRNA encoding the peptide. Thus, measuring mRNA is the obligated first step in investigating how expression of a gene can be regulated.

Keywords

Reverse Transcriptase Polymerase Chain Reaction Fluorescence Resonance Energy Transfer Molecular Beacon RNase Protection Assay cRNA Probe 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

9.7Glossary of Terms and Acronyms

ATP:

adenosine triphosphate

cDNA:

complementary DNA

cRNA:

complementary RNA

CsCL:

cesium chloride

Ct:

threshold cycle

DNA:

deoxyribonucleic acid

dNTP:

deoxynucleotide triphosphate

FRET:

fluorescence resonance energy transfer

GAPDH:

glyceral-dehyde-3-phosphate-dehydrogenase

GTC:

guanidinium isothiocyanate

hnRNA:

heterogeneous nuclear RNA

mRNA:

messenger RNA

oligo-dT:

oligonucleotide chain of deoxythimidines

PCR:

polymerase chain reaction

poly-A:

poly-adenylated

RNA:

ribonucleic acid

rRNA:

ribosomal RNA

RT-PCR:

reverse transcriptase polymerase chain reaction

UTP:

uridine triphosphate

Bibliography

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References

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    Real-time PCR goes prime time. TechNotes 8:1. Foster City, CA: Applied Biosystems, 2001.Google Scholar
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    SYBR® Green PCR and RT-PCR Reagents Protocol. Foster City, CA: Applied Biosystems, 2001.Google Scholar
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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  1. 1.Department of Cell Biology and AnatomyUniversity of South Carolina School of MedicineColumbiaUSA
  2. 2.Department of BiologyUniversity of Saskatchewan College of Arts and ScienceSaskatoonCanada

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