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Techniques for DNA Analysis

  • Javier S. Castresana
  • Paula Lázcoz
Chapter

Molecular biology is a dynamic field with techniques and analytical tools continuously being developed. Many of the fundamental DNA analysis techniques were developed more than 30 years ago and have evolved through various modifications, automation, and computerization. This chapter reviews the basic concepts and techniques in order to understand how the procedures progressed into those used today.

Keywords

Polymerase Chain Reaction Restriction Fragment Length Polymorphism Bacterial Artificial Chromosome Comparative Genomic Hybridization Methylation Specific Polymerase Chain Reaction 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

8. 13Glossary of Terms and Acronyms

32P:

radioactive isotope

5α-DHT:

5α–dihydrotestosterone

BAC:

bacterial artificial chromosome

BSP:

bisulfite sequencing PCR

CGH:

comparative genomic hybridization

CMC:

chemical mismatch cleavage

dATP:

deoxyadenosine triphosphate

dCTP:

deoxycytidine triphosphate

ddATP:

dideoxyadenosine triphosphate

ddCTP:

dideoxycytidine triphosphate

ddGTP:

dideoxyguanosine triphosphate

ddNTP:

dideoxynucleoside triphosphate

ddTTP:

dideoxythymidine triphosphate

DGGE:

denaturing gradient gel electrophoresis

dGTP:

deoxyguanosine triphosphate

DNA:

deoxyribonucleic acid

DNMT:

DNA methyltransferases

dNTP:

deoxynucleoside triphosphate

dsDNA:

double stranded DNA

dTTP:

deoxythymidine triphosphate

dUTP:

deoxyuridine triphosphate

EMC:

enzyme mismatch cleavage

FISH:

fluorescence in-situ hybridization

KCL:

potassium chloride

MAS:

marker-assisted selection

mRNA:

messenger RNA

MSP:

methylation specific PCR

NaOH:

sodium hydroxide

PAGE:

polyacrilamide gel electrophoresis

PCR:

polymerase chain reaction

PTT:

protein truncation test

qPCR:

quantitative real time polymerase chain reaction

RFLP:

restriction fragment length polymorphism

RNA:

ribonucleic acid

RT-PCR:

reverse transcription polymerase chain reaction

SDS-PAGE:

sodium dodecyl sulfate polyacrilamide gel electrophoresis

SSCP:

single-stranded conformational polymorphism

TGGE:

temperature gradient gel electrophoresis

tRNA:

transfer RNA

UV:

ultra-violet

VNTR:

variable number tandem repeats

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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  1. 1.Brain Tumor Biology UnitUniversity of NavarraPamplonaSpain
  2. 2.Department of Health SciencesUniversity of NavarraPamplonaSpain

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