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Recombinant DNA Technology

  • Pedro J. Chedrese
Chapter

The terms recombinant DNA technology, DNA cloning, molecular cloning, or gene cloning all refer to use of molecular techniques to select a specific sequence or sequences of DNA from an organism and transfer it into another organism to code for or alter specific traits. Thus, recombinant DNA technology provides a powerful molecular tool that enables scientists to engineer sequences of DNA.

Keywords

Haemophilus Influenzae Cloning Vector Stable Cell Line Inner Cell Mass Growth Hormone Gene 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

7.6Glossary of Terms and Acronyms

ampr:

ampicillin resistance gene

ATP:

adenosine triphosphate

BAC:

bacterial artificial chromosome

Ca2+:

calcium ion

CaCl2:

calcium chloride

cDNA:

complementary DNA

Cl:

chlorine ion

CO2:

carbon dioxide

DNA:

deoxyribonucleic acid

ES:

embryonic stem cells

GH:

growth hormone

HAC:

human artificial chromosome

HPO42–:

monohydrogen phosphate ion

ICM:

inner cell mass

IPTG:

isopropyl-β-D thiogalactopyranoside

K+:

potassium ion

LacZ:

β-galactosidase gene of E. coli

MCS:

multiple cloning sites also called polylinkers

Mg2+:

magnesium ion

mRNA:

messenger RNA

Na+:

sodium ion

NH4+:

ammonium

RNA:

ribonucleic acid

SO42–:

sulfate ion

YAC:

yeast artificial chromosome

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Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  1. 1.Department of BiologyUniversity of Saskatchewan College of Arts and ScienceSaskatoonCanada

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